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Registros recuperados: 29
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New information on the ecology of deep-sea vent communities in the azores triple junction area : preliminery results of the Diva 2 cruise (May 31-July 4, 1994) ArchiMer
Desbruyeres, Daniel; Alayse, Anne-marie; Antoine, Elisabeth; Barbier, Georges; Barriga, Fjas; Biscoito, Manuel; Briand, Patrick; Brulport, Jean-pierre; Comtet, Thierry; Cornec, Laurence; Crassous, Philippe; Dando, Paul; Fabri, Marie-claire; Felbeck, Horst; Lallier, Francois; Fiala Medioni, Aline; Goncalves, Joao; Menard, Frederic; Kerdoncuff, Jacques; Patching, John; Saldanha, Luis; Sarradin, Pierre-marie.
Tipo: Text
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00179/29005/27432.pdf
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Particle flux in the Rainbow hydrothermal vent field (Mid-Atlantic Ridge): Dynamics, mineral and biological composition ArchiMer
Khripounoff, Alexis; Vangriesheim, Annick; Crassous, Philippe; Segonzac, Michel; Colaco, Ana; Desbruyeres, Daniel; Barthelemy, Roxane.
In order to provide information about the export and the distribution of hydrothermal particulate material to the surrounding deep ocean, four moorings were deployed in the vicinity of the hydrothermal Rainbow vent field (Mid-Atlantic Ridge, 36 degrees 14'N, 2250 m depth). The first mooring was a sediment trap with a current meter deployed at 2 m from a chimney of the Rainbow vent field and 1.5 m above the bottom (a.b.) for 16 days. It represented the reference for the initial composition of particles produced by the vent. The total mean mass particle flux (6.9 g m(-2) d(-1)) was distinctly higher than the flux measured at the shallower hydrothermal vents on the MAR segment. This particulate flux showed a high temporal variation at the scale of a few days...
Tipo: Text Palavras-chave: Zooplankton; Biochemistry; Geochemistry; Particle flux; Hydrothermal vent.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-801.pdf
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Prorocentrum rivalis sp nov (Dinophyceae) and its phylogenetic affinities inferred from analysis of a mixed morphological and LSU rRNA data set ArchiMer
Delmail, David; Labrousse, Pascal; Crassous, Philippe; Hourdin, Philippe; Guri, Mathieu; Botineau, Michel.
A new freshwater epiphytic Prorocentrum species, Prorocentrum rivalis, from the temperate region of the Haute-Vienne, France, is described. This species is the third freshwater species identified among approximately 60 marine Prorocentrum species. This new species is described using scanning electron microscope and phylogenetic analyses by a polyphasic approach (LSU rRNA sequences combined with 9 morphological characters). The phylogenetic analysis attests that P. rivalis is close to other planktonic freshwater species and the freshwater Prorocentrum clade is evolutionarily derived from an epiphytic freshwater prorocentroid ancestor. The unique marine species in the freshwater clade results from an ecophysiological reversion. P. rivalis differs from other...
Tipo: Text Palavras-chave: Prorocentrum rivalis; Epiphytic microalgae; LSU; Phylogeny; Polyphasic approach; SEM.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00035/14601/12235.pdf
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Direct observation of intense turbidity current activity in the Zaire submarine valley at 4000 m water depth ArchiMer
Khripounoff, Alexis; Vangriesheim, Annick; Babonneau, Nathalie; Crassous, Philippe; Dennielou, Bernard; Savoye, Bruno.
A large turbidity current was detected in the Zaire submarine valley at 4000 m water depth. Current meters, turbidimeter and sediment trap deployed on a mooring located in the channel axis, although they were damaged, recorded the signature of a very high energy event. An average velocity of more than 121 cm s-1 was measured 150 m above the channel floor. Coarse sand and plant debris were collected at 40 m height. The turbidity current clearly overflowed the edges of the valley as demonstrated by the large quantity of turbiditic material (464 mg organic carbon m-2 d-1) found in the sediment trap moored 13 km south from the channel axis.
Tipo: Text Palavras-chave: West equatorial Africa; Particle composition; Particle flux; Zaire channel; Turbidity current.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-1419.pdf
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Organic matter budget in the Southeast Atlantic continental margin close to the Congo Canyon: In situ measurements of sediment oxygen consumption ArchiMer
Rabouille, C.; Caprais, Jean-claude; Lansard, B.; Crassous, Philippe; Dedieu, K.; Reyss, J. L.; Khripounoff, Alexis.
A study of organic carbon mineralization from the Congo continental shelf to the abyssal plain through the Congo submarine channel and Angola Margin was undertaken using in situ measurements of sediment oxygen demand as a tracer of benthic carbon recycling. Two measurement techniques were coupled on a single autonomous platform: in situ benthic chambers and microelectrodes, which provided total and diffusive oxygen uptake as well as oxygen microdistributions in porewaters. In addition, sediment trap fluxes, sediment composition (Org-C, Tot-N, CaCO3, porosity) and radionuclide profiles provided measurements of, respectively input fluxes and burial rate of organic and inorganic compounds. The in situ results show that the oxygen consumption on this margin...
Tipo: Text Palavras-chave: Sediment carbon budget; Organic carbon burial; Congo Submarine Canyon; Organic carbon recycling in sediments; In situ oxygen demand.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7316.pdf
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Spatial and temporal patterns of benthic macrofaunal communities on the deep continental margin in the Gulf of Guinea ArchiMer
Galeron, Joelle; Menot, Lenaick; Renaud, Nolwenn; Crassous, Philippe; Khripounoff, Alexis; Treignier, C.; Sibuet, M..
Density, taxonomic composition at higher taxon level and vertical distribution of benthic macrofaunal communities and sediment characteristics (pore water, nitrogen, organic carbon, sulfur, C/N ratio, n-alcohol biomarkers) were examined at three deep sites on the Congo-Gabon continental margin. This study was part of the multidisciplinary BIOZAIRE project that aimed at studying the deep benthic ecosystems in the Gulf of Guinea. Sampling of macrofaunal communities and of sediment was conducted during three cruises (January 2001, December 2001 and December 2003) at two downslope sites (4000 m depth), one located near the Congo submarine channel (15 km in the south) and the other one far from the channel (150km in the South). The third area located 8 km north...
Tipo: Text Palavras-chave: Deep sea; Distribution; Community structure; Macrofauna; Congo canyon; Congo Angola margin; Equatorial west Africa margin; Atlantic.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7315.pdf
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Spatial heterogeneity of macrofaunal communities in and near a giant pockmark area in the deep Gulf of Guinea ArchiMer
Menot, Lenaick; Galeron, Joelle; Olu, Karine; Caprais, Jean-claude; Crassous, Philippe; Khripounoff, Alexis; Sibuet, Myriam.
The structure and familial-level composition of macrofaunal communities were examined within and at distance from the giant pockmark Regab in the Southern Gulf of Guinea, at 3200 m depth. The two main questions addressed in this study were (i) does habitat partitioning by large symbiont-bearing taxa. influence macrofaunal assemblages and their environment? and (ii) to what extent does the chemosynthesis-based ecosystem influence the structure and the composition of nearby macrobenthic communities? Along two radials, at sites from 250 to 1000 m away from the active centre of the cold seep, the abundance and composition of the macrofauna were typical of a deep-sea community at that depth. Except for a few cores sampled in or near dead vesicomyid fields at...
Tipo: Text Palavras-chave: Cold seep; Diversity; Gulf of Guinea; Habitat heterogeneity; Macrofauna; Open slope; Siboglinid tubeworms; Vesicomyid clams.
Ano: 2010 URL: https://archimer.ifremer.fr/doc/00002/11282/8099.pdf
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Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation? ArchiMer
Coutard, Francois; Crassous, Philippe; Droguet, Michael; Gobin, Eric; Colwell, Rita; Pommepuy, Monique; Hervio Heath, Dominique.
The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population...
Tipo: Text Palavras-chave: Transmission electron microscopy; Scanning electron microscopy; Resuscitation; Regrowth; Vibrio parahaemolyticus; VBNC state.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3117.pdf
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Acquisition of epibiotic bacteria along the life cycle of the hydrothermal shrimp Rimicaris exoculata ArchiMer
Guri, Mathieu; Durand, Lucile; Cueff-gauchard, Valerie; Zbinden, Magali; Crassous, Philippe; Shillito, Bruce; Cambon-bonavita, Marie-anne.
The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron...
Tipo: Text Palavras-chave: Symbiosis; Larvae; Methanotrophic symbiont; Rimicaris exoculata; Transmission pathways.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00072/18318/15997.pdf
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Spatial organization of a sedimentary macrobenthic community located on the West African Equatorial margin ArchiMer
Brind'Amour, Anik; Menot, Lenaick; Galeron, Joelle; Crassous, Philippe.
Multiscale variability of a macrobenthic community inhabiting fine sediments on the West African Equatorial margin was assessed during three cruises as part of the BIOZAIRE research program. Spatial scales ranged from 15 to 550 m (within station) and from 1000 to 7000 m (between stations). Principal Coordinates of Neighbour Matrices (PCNM) allowed the detection of multiscale patterns of variability within a location, while a binary coding of the sampling stations was used at broad spatial scales. Significant patterns were observed at 170 and >4000 m. The two spatial scales displayed similar biotic responses, with the Scaphopoda and Bivalvia having patterns that were opposite to the Pholoidae. A spatio-temporal patchwork of environmental variables might...
Tipo: Text Palavras-chave: Spatial distribution; PCNM; Multiscale analyses; Macrobenthic communities; Habitat patches; BIOZAIRE.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6508.pdf
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Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease ArchiMer
Trinkler, Nolwenn; Bardeau, Jean-francois; Marin, Frederic; Labonne, Maylis; Jolivet, Aurelie; Crassous, Philippe; Paillard, Christine.
The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be...
Tipo: Text Palavras-chave: Aragonite; Hemocytes; Shell microstructure; Calcium carbonate; Biomineralization; Mollusc; Raman microspectrometry; Scanning electron microscopy.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00029/14065/11301.pdf
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Colonization patterns along the equatorial West African margin: Implications for functioning and diversity maintenance of bathyal and abyssal communities ArchiMer
Menot, Lenaick; Crassous, Philippe; Desbruyeres, Daniel; Galeron, Joelle; Khripounoff, Alexis; Sibuet, M..
In the framework of the deep-sea environmental programme BIOZAIRE (Ifremer-Total), colonization trays were deployed for 283-433 days at three sites along the equatorial West African margin: ZA at 1300-m depth, ZC at 4000-m depth far from the Congo canyon and ZD at 4000-m depth close to the Congo canyon. The experiments aimed at determining the influence of depth and local environmental settings on macrofaunal colonization patterns and organic carbon degradation rates. The trays were filled with glass beads and this artificial substrate was enriched with ground particulate organic matter in a gradient of 0%, 0.34%, 1.02% and 3.43% organic carbon. The highest rates of organic carbon degradation ranged, according to the duration of the experiments, from 1.59...
Tipo: Text Palavras-chave: Diversity; Organic enrichment; Macrofauna; Deep sea; Colonization; Biozaire.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7323.pdf
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Detection of polycyclic aromatic hydrocarbon (PAH) compounds in artificial sea-water using surface-enhanced Raman scattering (SERS) ArchiMer
Peron, Olivier; Rinnert, Emmanuel; Lehaitre, Michel; Crassous, Philippe; Compere, Chantal.
This paper reports an accurate synthesis of surface-enhanced Raman scattering (SERS) active substrates, based on gold colloidal monolayer, suitable for in situ environmental analysis. Quartz substrates were functionalized by silanization with (3-mercaptopropyl)trimethoxysilane (MPMS) or (3-aminopropyl)trimethoxysilane (APTMS) and they subsequently reacted with colloidal suspension of gold metal nanoparticles: respectively, the functional groups SH and NH2 bound gold nanoparticles. Gold nanoparticles were prepared by the chemical reduction of HAuCl4 using sodium tricitrate and immobilized onto silanized quartz substrates. Active substrate surface morphology was characterized with scanning electron microscopy (SEM) measurements and gold nanoparticles...
Tipo: Text Palavras-chave: Sensor; Sea water; PAH detection; SERS; Silanization; Gold nanoparticles.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6616.pdf
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Remises en suspension sédimentaires observées en Méditerranée par 2 000 m de profondeur à l'aide de pièces à particules ArchiMer
Crassous, Philippe; Khripounoff, Alexis; Larosa, Jacques; Miquel, Juan-carlos.
Four sediment traps of two different types and two current meters were deployed fixed to two identical moorings, at a depth of 2 000 m, off the coast at Nice, for 70 days between April and June 1987. The particulate flux measured with sediment traps decreased from 1 000 to 95 mg/m2/day during this experiment. The carbonate carbon concentration of the particles also decreased during this period; organic carbon concentrations varied inversely with the flux, increasing from 1.8 to 3.7 % of the particle dry weight. It is concluded that these changes reveal the presence of a deep-sea storm with a significant resuspension of sediment to a height greater than 200 m (distance of the traps from the bottom). By the end of the experiment, identical sediment traps had...
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/00101/21244/18859.pdf
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Variation horizontale du flux particulaire à micro- et méso-échelle dans la plaine abyssale de Californie (4 100 m de profondeur) ArchiMer
Crassous, Philippe; Khripounoff, Alexis.
Nine sediment traps were assembled in triplicate on a frame and deployed at 100 m above the bottom in the California abyssal plain (4 100 m depth) for ten days. The distances between traps were from 30 cm to 11 km. The main flux measured during this period was 167 mg m(-2) day(-1) with a minimum of 43 mg m(-2) d(-1) and a maximum of 283 mg m(-2) d(-1). This high variation of the particulate flux at the kilometric scale was also observed at a metric scale: for example, the particulate flux measured with one of triplicate traps was equal to 82.3 +/- 46.5 mg m(-2) d(-1) (more than 50 % of variation) between traps distant of less than 1 m. To explain this heterogeneity of the flux measurement, some hypotheses are developed in this study: sampling artefact,...
Tipo: Text Palavras-chave: SEDIMENT TRAPS; DEEP SEA; HORIZONTAL FLUX VARIATION.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00098/20885/18499.pdf
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Temporal variation of currents, particulate flux and organism supply at two deep-sea hydrothermal fields of the Azores Triple Junction ArchiMer
Khripounoff, Alexis; Vangriesheim, Annick; Crassous, Philippe; Segonzac, Michel; Lafon, V; Waren, A.
In order to provide information on the production and the distribution of hydrothermal particulate and biological material produced by deep-sea hydrothermal vents, three moorings with sediment traps and currentmeters were deployed for one year in the Azores Triple Junction region. Two moorings were positioned close to two hydrothermal vent fields at the Mid-Atlantic Ridge (Rainbow, 36 degrees 14'N, 33 degrees 54'W, 2250 in depth and Lucky Strike 37 degrees 17'N, 32 degrees 17'W, 1600 in depth). A third was moored out of the influence of the Rainbow vents representing the reference of the pelagic flux in this area. At the Rainbow field, particulate flux (range 341-634 g m(-2)d(-1)) showed a high temporal variation controlled essentially by the current...
Tipo: Text Palavras-chave: Bivalve reproduction; Bivalve larvae; Particle composition; Particle flux; Currents; Deep sea hydrothermal vents.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4059.pdf
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A novel mcl-PHA produced on coprah oil by Pseudomonas guezennei biovar. tikehau, isolated from a "kopara" mat of French Polynesia ArchiMer
Simon-colin, Christelle; Raguenes, Gerard; Crassous, Philippe; Moppert, X; Guezennec, Jean.
Pseudomonasguezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was followed along 52 h by regular culture sampling. The polyester was purified from freeze-dried cells and analysed by nuclear magnetic resonance (NMR), Fourier transform infra red (FTIR), and gas chromatography mass spectrometries. The copolyester produced by P. guezennei biovar. tikehau from coprah oil mainly consisted of saturated monomers, i.e. 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), and the monomeric composition of the polyester did not change...
Tipo: Text Palavras-chave: Polyhydroxyalkanaote; PHAs; Pseudomonas; "kopara"; Microbial mat.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6119.pdf
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Sedimentological imprint on subseafloor microbial communities in Western Mediterranean Sea Quaternary sediments ArchiMer
Ciobanu, Maria Cristina; Rabineau, Marina; Droz, Laurence; Revillon, Sidonie; Ghiglione, J. -f.; Dennielou, Bernard; Jorry, Stephan; Kallmeyer, J.; Etoubleau, Joel; Pignet, Patricia; Crassous, Philippe; Vandenabeele-trambouze, Odile; Laugier, J.; Guegan, Matthieu; Godfroy, Anne; Alain, Karine.
An interdisciplinary study was conducted to evaluate the relationship between geological and paleoenvironmental parameters and the bacterial and archaeal community structure of two contrasting subseafloor sites in the Western Mediterranean Sea (Ligurian Sea and Gulf of Lion). Both depositional environments in this area are well-documented from paleoclimatic and paleooceanographic point of views. Available data sets allowed us to calibrate the investigated cores with reference and dated cores previously collected in the same area, and notably correlated to Quaternary climate variations. DNA-based fingerprints showed that the archaeal diversity was composed by one group, Miscellaneous Crenarchaeotic Group (MCG), within the Gulf of Lion sediments and of nine...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00090/20171/17826.pdf
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Nautilia abyssi sp nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent ArchiMer
Alain, Karine; Callac, Nolwenn; Guegan, Marianne; Lesongeur, Francoise; Crassous, Philippe; Cambon-bonavita, Marie-anne; Querellou, Joel; Prieur, Daniel.
A novel strictly anaerobic, thermophilic, sulfur-reducing bacterium, designated PH1209(T), was isolated from an East Pacific Rise hydrothermal vent (13 degrees N) sample and studied using a polyphasic taxonomic approach. Cells were Gram-negative, motile rods (approx. 1.60x0.40 mu m) with a single polar flagellum. Strain PH1209(T) grew at temperatures between 33 and 65 degrees C (optimum 60 degrees C), from pH 5.0 to 8.0 (optimum 6.0-6.5), and between 2 and 4% (w/v) NaCl (optimum 3%). Cells grew chemolithoautotrophically with H-2 as an energy source, So as an electron acceptor and CO2 as a carbon source. Strain PH1209(T) was also able to use peptone and yeast extract as carbon sources. The G+C content of the genomic DNA was 35 mol%. Phylogenetic: analyses...
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6585.pdf
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Different types of sediment gravity flows detected in the Var submarine canyon (northwestern Mediterranean Sea) ArchiMer
Khripounoff, Alexis; Crassous, Philippe; Lo Bue, N.; Dennielou, Bernard; Silva Jacinto, Ricardo.
Current velocities and vertical sediment fluxes in the Var submarine canyon were assessed at three stations respectively at 800 m, 1200 m and 1800 m depth, using moorings deployed for 4 months during winter 2008-2009. During this period, we observed three major sediment gravity flows, all characterized by sudden increases in current velocity that lasted 2-5 h and by downward particle fluxes. Each gravity flow, described using a high frequency current meter and two Acoustic Doppler Current Profiler (75 and 300 kHz ADCP) showed distinctive features. The first event, triggered during a flood of the Var River, was determined to be a hyperpycnal current with a large vertical extent (>100 m high) and relatively low velocity (40 cm s(-1)). The second event,...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00113/22394/20343.pdf
Registros recuperados: 29
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