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Sensitivity of aquatic organisms to ethanol and its potential use as bioindicators Biological Sciences
Silva, Adilson Ferreira; Cruz, Claudinei da; Garlich, Nathalia; Cerveira Junior, Wilson Roberto; Pitelli, Robinson Antonio.
The aim of this research was to evaluate the feasibility for the use of the organisms Lemna minor, Azolla caroliniana, Hyphessobrycon eques, Pomacea canaliculata and Daphnia magna as exposure bioindicators for ethanol (lethal and effective concentration 50% - LC50(I)/EC50(I)). Thus, the following concentrations were used 5.0; 10.0; 20.0; 30.0; 40.0 and 50.0 mg L-1 ethanol on L. minor; 25.0; 50.0; 75.0; 100.0; 150.0 and 200.0 mg L-1 on A. caroliniana; 0.3; 0.5; 1.0; 2.0 and 3.0 mg L-1 on H. eques; 0.05; 0.10; 0.20; 0.40 and 0.80 mg L-1 on P. canaliculata; and 40.0; 60.0; 80.0; 100.0; 120.0 and 140.0 mg L-1 on D. magna. An untreated control was also kept for all organisms, with three repetitions. The increase in the ethanol concentration elevated the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Recursos pesqueiros e pesca environmental impact; LC50; Ecology ecotoxicologia sentinel organisms environmental impact ecology Ecotoxicologia.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28946
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Copper sulfate acute ecotoxicity and environmental risk for tropical fish Biological Sciences
Silva, Adilson Ferreira da; Cruz, Claudinei da; Rezende, Flavia Regina Lazaro de; Yamauchi, Alfredo Kohiti Feres; Pitelli, Robinson Antonio.
The aim of this study was to estimate copper sulfate acute toxicity and to determine death percentage and environmental risk on guppy fish (Phallocerus caudimaculatus), zebrafish (Brachydanio rerio), mato grosso (Hyphessobrycon eques), and pacu (Piaractus mesopotamicus). Fish were exposed to 0.01, 0.03, 0.05, 0.07, 0.10, and 0.30 mg L-1 (guppy), 0.05, 0.07, 0.10, and 0.30 mg L-1 (zebrafish), 0.07, 0.10, 0.20, and 0.30 mg L-1 (mato grosso) and 9.5, 10.0, 10.5, 11.0, 11.5, and 12.0 mg L-1 (pacu) of copper sulfate, with triplicate control. The estimated 50% average lethal concentrations (LC50; 96 hours) were 0.05 (guppy), 0.13 (zebrafish); 0.16 (mato grosso) and 10.36 mg L-1 (pacu). Copper sulfate was extremely toxic for guppy, highly toxic for zebrafish and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.00.00.00-6 - Ciências Biológicas bioindicators; Algaecide; Toxicity 2.05.03.00-8.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18373
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Isolation of Saprolegnia aenigmatica oomycetes and protocol for experimental infection of pacu (Piaractus mesopotamicus) Biological Sciences
Carraschi, Silvia Patrícia; Garlich, Nathália; Souza-Pollo, Andressa; Pereira, Daniela Isabel Brayer; Cruz, Claudinei da; Ranzani-Paiva, Maria José Tavares.
 Successful disease treatment depends on molecular studies under indoor conditions with experimental infection protocols that facilitate understanding the disease and the drug`s efficacy. The internal transcribed spacer (ITS) region was sequenced from three isolates, which were identified as Saprolegnia aenigmatica. Subsequently, healthy fish were immunosuppressed with dexamethasone (1.2 mg kg-1) and descaled to the skin using a sharp scalpel. These individuals were isolated in individual aquariums maintained at 22°C. Individuals in one group were subcutaneously inoculated with 9,000 zoospores (DDZ treatment), a second group was exposed to oomycetes in water with three colonized baits (DDB), a third group was maintained in water without zoospores (DD), and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.00-0 oomycete; Zoosporangium; Oogonium; Saprolegniasis. Aquicultura.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/38186
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