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| Angrimani,D.S.R.; Nichi,M.; Brito,M.M.; Kawai,G.K.V.; Rui,B.R.; Losano,J.D.A.; Vieira,N.M.G.; Francischini,M.C.P.; Cruz,D.S.G.; Queiroz-Hazarbassanov,N.; Massoco,C.O.; Vannucchi,C.I.. |
| ABSTRACT The aim of this study was to evaluate the effect of supplementation with different concentrations of reduced glutathione GSH (0; 5; 7.5; 10mM) in the extender for cryopreservation in dogs with evaluations performed after glycerolization (chilled) and thawing (thawed). For this purpose, we used 8 dogs and two semen collections were performed in a weekly interval, totaling 16 semen samples. The sperm were analyzed by automatic sperm motility (CASA) and flow cytometry analysis of mitochondrial potential (JC1 dye) and membrane/acrosome integrity (FITC-PI dyes). We evaluated subjectively the membrane and acrosome integrity, mitochondrial activity and DNA integrity. Seminal plasma was evaluated for lipid peroxidation (TBARS concentration). Chilled and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reactive oxygen species; Cryopreservation; Antioxidant; Canine. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200419 |
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