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Multiple gene sequence analysis using genes of the bacterial DNA repair pathway BABT
Rotelok Neto,Miguel; Galvão,Carolina Weigert; Cruz,Leonardo Magalhães; Guizelini,Dieval; Silva,Leilane Caline; Garcia,Jarem Raul; Etto,Rafael Mazer.
The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways. Despite the amount of available genes in public database, the usual method to place genomes in a taxonomic context is based mainly on the 16S rRNA or housekeeping genes. Thus, the relationships among genomes remain poorly understood. In this work, an approach of multiple gene sequence analysis based on genes of DNA repair pathway was used to compare bacterial genomes. Housekeeping and DNA repair genes were searched in 872 completely sequenced bacterial genomes. Seven DNA repair and housekeeping genes from distinct metabolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial genome; Multiple gene sequence analysis; Taxonomic assignment; DNA repair genes; HGT.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300421
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Draft genome sequence of Paraburkholderia tropica Ppe8 strain, a sugarcane endophytic diazotrophic bacterium BJM
Silva,Paula Renata Alves da; Simões-Araújo,Jean Luiz; Vidal,Márcia Soares; Cruz,Leonardo Magalhães; Souza,Emanuel Maltempi de; Baldani,José Ivo.
Abstract Paraburkholderia tropica (syn Burkholderia tropica) are nitrogen-fixing bacteria commonly found in sugarcane. The Paraburkholderia tropica strain Ppe8 is part of the sugarcane inoculant consortium that has a beneficial effect on yield. Here, we report a draft genome sequence of this strain elucidating the mechanisms involved in its interaction mainly with Poaceae. A genome size of approximately 8.75 Mb containing 7844 protein coding genes distributed in 526 subsystems was de novo assembled with ABySS and annotated by RAST. Genes related to the nitrogen fixation process, the secretion systems (I, II, III, IV, and VI), and related to a variety of metabolic traits, such as metabolism of carbohydrates, amino acids, vitamins, and proteins, were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diazotrophic; Next generation sequencing; Nitrogen-fixing; Secretion system.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200210
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