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	Provedor de dados Electron. J. Biotechnol. (3) Autor Díaz,Mauricio (3) Arenas,Gloria (2) Marshall,Sergio H (2) Marchetti,Stefano (1) Peña-Cortés,Hugo (1) Polanco,Victor (1) Ramírez,Ingrid (1) Venturini,Elena (1) Mais... Palavra-chave 12-oxophytodienoate reductase (1) Antimicrobial (1) Antimicrobial peptides (1) Cecropin (1) Escherichia coli (1) Expression (1) Fusion (1) Intein (1) Jasmonic acid (1) OPDA (1) Peptide (1) Solanum tuberosum (1) Soluble (1) Stress (1) Wounding (1) Mais... Tipo do documento Journal article (3) Ano 2012 (2) 2008 (1) País Chile (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Intein-mediated expression of cecropin in Escherichia coli Electron. J. Biotechnol. Díaz,Mauricio; Venturini,Elena; Marchetti,Stefano; Arenas,Gloria; Marshall,Sergio H. Different strategies have been used to overcome the difficulties to produce antimicrobial peptides. Here we used Intein Mediated Purification with an Affinity Chitin-binding Tag (IMPACT-System, New England Biolabs) for the expression of the antimicrobial peptide cecropin to reduce its sensitivity to intracellular proteases and use its inducible self-cleaving capability to remove the carrier. Cecropin was cloned into suitable expression vector pTYB11, and expression induced by IPTG in Escherichia coli ER2566. The use of 22ºC induction allowed the expression of cecropin with its intein carrier in soluble form. Cell extracts were purified by chitin affinity chromatography and intein-mediated splicing of the target protein was achieved by thiol addition,... Tipo: Journal article Palavras-chave: Antimicrobial; Cecropin; Fusion; Intein; Peptide; Soluble. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200003 Design and expression of a retro doublet of cecropin with enhanced activity Electron. J. Biotechnol. Díaz,Mauricio; Arenas,Gloria; Marshall,Sergio H. Novel doublet molecules of cecropin A from Drosophila melanogaster were designed and constructed combining the regular (CECdir) with the inverted (CECret) coding sequence of the standard CEC A1 gene resulting in the following configurations: CECdir-CECret and CECret-CECdir. These two recombinant molecules were generated using a three-primer driven PCR reaction yielding composite single functional aminoacidic molecules with the coding sequences of CECdir linked in frame with the coding sequence of CECret and vice versa. In order to obtain these constructions, a retropeptide DNA-coding sequence was chemically synthesized to match the expected polarity of the newly generated CECret sequence. Both doublet antimicrobial peptides (drAMPs) were cloned in the T7... Tipo: Journal article Palavras-chave: Antimicrobial peptides; Escherichia coli; Expression. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200006 Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum Electron. J. Biotechnol. Díaz,Mauricio; Polanco,Victor; Ramírez,Ingrid; Peña-Cortés,Hugo. Jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) are signal molecules involved in the stress and defense responses in plants. A full-length cDNA clon of OPR3 encoding 12-oxophytodienoate reductase 3, key enzyme involved in the biosynthesis of JA from linolenic acid was obtained from a Solanum tuberosum cDNA library. Sequence analysis showed that OPR3 encoded a polypeptide of 400 amino acids with a predicted molecular mass of 43.9 kDa and pI of 7.72. The deduced amino acid sequence of OPR3 showed high similarities to other 12-oxophytodienoate reductases. A peroxisomal signal sequence indicates OPR3 probable location in peroxisome. Levels of OPR3 mRNA accumulated in potato leaves reaching maximum levels within 1 hr of mechanical wounding. Elevated... Tipo: Journal article Palavras-chave: 12-oxophytodienoate reductase; Jasmonic acid; OPDA; Solanum tuberosum; Stress; Wounding. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100010 Registros recuperados: 3 Primeira ... 1 ... Última

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