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| GALLARDO-ESCÁRATE,CRISTIAN; ÁLVAREZ-BORREGO,JOSUÉ; VON BRAND,ELISABETH; DUPRÉ,ENRIQUE; DEL RÍO-PORTILLA,MIGUEL ÁNGEL. |
| In observations by confocal or conventional fluorescence microscopy, important factors should be considered in order to obtain accurate images. One of them, such as the fluorescence bleaching from highest intensity to lowest signal of fluorescence is a common problem with several DNA fluorochromes and especially for DAPI stain. The fluorescence of DAPI fades rapidly when it is exposed to UV light, under optimal conditions of observation. Although the fading process can be retarded using a mounting medium with antifading reagents, the photochemical process underlying the fluorescence decay has not yet been fully explained. In addition, no relationship between fluorescence fading and nuclear DNA content has been tested. In order to test this relationship, we... |
| Tipo: Journal article |
Palavras-chave: Nuclear DNA content; Fluorescence fading; Image analysis; DAPI. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100004 |
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| DUPRÉ,ENRIQUE; FLORES,LUIS; PALMA,SERGIO. |
| The first description of the larval stages of a representative of the family Rhynchocinetidae from the southeastern Pacific coast of South América is presented. Larvae of the rock shrimp Rhynchocinetes typus, from rocky subtidal environment of the Chile-Pera coast were reared in the laboratory at 22 °C, salinity 32 and feeding with Anemia franciscana. Seven zoeal stages, which occur through 10 successive moults, are described and illustrated in detail. Morphological comparison made between R. typus and other congeneric and related species belonging to Rhynchocinetid showed the main characteristic for the diagnosis of zoeal stages are: (a) the pereiopods appear in the third stage, (b) the exopod of the antennae have six segments, and (c) no pleopods were... |
| Tipo: Journal article |
Palavras-chave: Larval development; Shrimp; Rhynchocinetes; Caridea. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2008000200001 |
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