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Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers R. Bras. Zootec.
Weiler,Roberto Luis; Dall’Agnol,Miguel; Simioni,Carine; Krycki,Karine Cristina; Dahmer,Nair; Guerra,Divanilde.
ABSTRACT The aim of this study was to determine the mode of reproduction of a hybrid progeny derived from intraspecific crosses of Paspalum notatum through cytoembryological analysis and use of RAPD (random amplification of polymorphic DNA) molecular markers. Cytoembryological analysis allowed identification of the mode of reproduction of 28 plants that were selected after agronomic productivity evaluations. Of these, 19 had embryo sac morphology compatible with an apomictic mode of reproduction and nine had embryo sac morphology compatible with a sexual mode of reproduction. Meanwhile, molecular marker analysis for 194 individuals showed 54 sexual and 140 apomictic plants; of the 28 plants analyzed by the two methods, ten results (35.7%) were in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apomixis; Molecular analysis; Paspalum notatum; Plant breeding; RAPD.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000300185
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Cytogenetic data for Paspalum notatum Flügge accessions Scientia Agricola
Dahmer,Nair; Schifino-Wittmann,Maria Teresa; Dall'Agnol,Miguel; Castro,Biane de.
Several species of the genus Paspalum L. are important forages, due their to quality, productivity and tolerance to environmental stresses. Chromosome numbers, meiotic configurations and pollen fertility were evaluated in a collection of 85 accessions of Paspalum notatum Flügge and in seven accessions of Pensacola (P. notatum var saurae). All P. notatum accessions were tetraploid, with 2n = 4x = 40, except one diploid accession, considered as an escape of Pensacola. All Pensacola plants had 2n = 2x = 20. Meiotic configurations at diakinesis and metaphase I varied among tetraploid accessions, from plants with only bivalents to plants with high frequency of quadrivalents. Pollen fertility varied from 82.5 to 95.9% among diploid accessions and from 72.4 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apomixis; Chromosome numbers; Meiotic configurations; Plant breeding; Pollen fertility.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000400009
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