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| Registros recuperados: 10 | |
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| Jensen, A.N.; Andersen, M.T.; Dalsgaard, A.; Baggesen, D.L.; Nielsen, E.M.. |
| Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for species-specific identification of C. jejuni, C. coli, C. lari, C. upsaliensis and of a hipO gene nucleotide probe for detection of C. jejuni by colony-blot hybridization were determined by testing a total of 75 reference strains of Campylobacter spp. and related organisms. The rt-PCR method allowed species-specific detection of Campylobacter spp. in naturally infected pig faecal samples after an enrichment step, whereas the hybridization approach enhanced the specific isolation of C. jejuni (present in... |
| Tipo: Journal paper |
Palavras-chave: Pigs. |
| Ano: 2005 |
URL: http://orgprints.org/4804/1/4804.pdf |
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| Holmqvist, A.; Møller, J.; Dalsgaard, A.. |
| Thermophilic composting of faecal matter from urine diverting toilets can effectively reduce the numbers of faecal bacterial indicators and pathogen. Already at 50C, the numbers of pathogens, including the Salmonella phage, and indicator organisms analysed were effectively reduced within a few days of exposure. Although the numbers of enterococci were reduced, they were continuous isolated as purple colonies on Slanetz and Bartleys agar after prolonged exposure at all temperature levels studied. This indicates that certain micro-organisms present in the composted faecal material, Enterococcus spp. or micro-organisms resembling enterococci on the agar medium, can survive and multiply even at 60C. These findings question the use of enterococci as faecal... |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Recycling; Balancing and resource management. |
| Ano: 2003 |
URL: http://orgprints.org/2087/1/HMDPoster1.pdf |
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| Møller, J.; Forslund, A.; Dalsgaard, A.. |
| Large variations in numbers of faecal indicator bacteria were found irrespective of the storage time of collected human faeces. Little heat seemed generated from composting processes when bin units were stored locally in households. The low reduction in microbiological parameters and very limited temperature increase were generally corroborated by the results obtained in experiment 2 when pathogen indicators were added to thoroughly mixed faecal matter. Even though Salmonella died of rapidly the other faecal bacterial indicators survived in large numbers. We conclude, that the collection and storage of human faeces in the closed plastic bins studied here is associated with only little temperature increase and subsequent reduction in faecal bacterial... |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Recycling; Balancing and resource management. |
| Ano: 2003 |
URL: http://orgprints.org/2088/1/MFDposter2.pdf |
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| Hackl, E.; Sessitsch, A.; Arthurson, V.; Baggesen, D.L.; Dalsgaard, A.; Friedel, J.K.; Hartmann, A.; Koller, M.; van Bruggen, A.H.C.; Widmer, F.; Wyss, G.; Zijlstra, C.A.. |
| PathOrganic assesses risks associated with the consumption of fresh and minimally processed vegetables due to the prevalence of bacterial human pathogens (e.g. Salmonella enterica, pathogenic E. coli, Campylobacter spp., Listeria monocytogenes) in organically grown plant produce. The project aims at evaluating whether organic production poses a risk on food safety and addresses the food chain by taking into consideration potential sources of pathogen transmission (e.g. animal manure). In addition, it evaluates whether organic production may reduce the risk of pathogen manifestation. From a European perspective, vegetable-linked outbreaks are not well investigated. Within the PathOrganic project, surveys of organically grown vegetables are carried out in... |
| Tipo: Report |
Palavras-chave: Food security; Food quality and human health Vegetables. |
| Ano: 2012 |
URL: http://orgprints.org/21197/1/PathOrganic_final_report_OrgEprints.doc |
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| Jensen, A.N.; Dalsgaard, A.; Baggesen, D.L.; Nielsen, E.M.. |
| The occurrence and species distribution of thermophilic Campylobacter was investigated in organic outdoor pigs. An increased exposure of outdoor pigs to C. jejuni from the environment may cause a shift from a normal dominance of C. coli to more C. jejuni, which may imply a concern of reduced food safety. Bacteriological methods for determination of Campylobacter excretion level were combined with colony-blot hybridization and real-time PCR for specific detection of C. jejuni in pigs. Campylobacter was isolated from pigs (n = 47), paddock environment (n = 126) and wildlife (n = 44), identified to species by real-time PCR and sub-typed by serotyping (Penner) and pulse-field gel electrophorsis (PFGE) genotyping. All pigs excreted Campylobacter (103–107 CFU... |
| Tipo: Journal paper |
Palavras-chave: Food security; Food quality and human health Pigs. |
| Ano: 2006 |
URL: http://orgprints.org/9156/1/9156.pdf |
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| Møller, J.; Backlund, A.; Jørgensen, L.T.; Forslund, A.; Dalsgaard, A.. |
| Som resultat af projekterne kan drages følgende hovedkonklusioner om kompostering af fæces fra mennesker: Fæces fra de undersøgte typer af kompostbeholdere bør ikke anvendes til jordbrugsformål uden viderebehandling, da dette skønnes at være behæftet med hygiejne- og sundhedsrisici. Det skyldes, at der ikke blev dokumenteret egentlige termofile temperaturstigninger i fæcesmaterialet i komposttoiletenhederne i Hjortshøj, Dyssekilde og Sverige, og at fækale indikatorbakteriers antal varierede voldsomt, og der ingen entydig tendens var til forekomst af lavere kimtal ved lange opbevaringstider af de opsamlede fækalier. |
| Tipo: Report |
Palavras-chave: Recycling; Balancing and resource management. |
| Ano: 2003 |
URL: http://orgprints.org/1920/1/1920a.pdf |
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| Møller, J.; Backlund, A.; Jørgensen, L.T.; Forslund, A.; Dalsgaard, A.. |
| Som resultat af projekterne kan drages følgende hovedkonklusioner om kompostering af fæces fra mennesker: Fæces fra de undersøgte typer af kompostbeholdere bør ikke anvendes til jordbrugsformål uden viderebehandling, da dette skønnes at være behæftet med hygiejne- og sundhedsrisici. Det skyldes, at der ikke blev dokumenteret egentlige termofile temperaturstigninger i fæcesmaterialet i komposttoiletenhederne i Hjortshøj, Dyssekilde og Sverige, samt at fækale indikatorbakteriers antal varierede voldsomt, og der ingen entydig tendens var til forekomst af lavere kimtal ved lange opbevaringstider af de opsamlede fækalier. |
| Tipo: Report |
Palavras-chave: Recycling; Balancing and resource management. |
| Ano: 2002 |
URL: http://orgprints.org/2086/1/Faeceskompostrapport.pdf |
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| Arthurson, V.; Baggesen, D.; Brankatschk, K.; Dalsgaard, A.; Duffy, B.; Fenzl, C.; Friedel, J.K.; Hackl, E.; Hartmann, A.; Hedin, F.; Hofmann, A.; Jäderlund, L.; Jansson, J.; Jensen, A.N.; Koller, M.; Mäder, P.; Rinnofner, T.; Schmid, M.; Storm, C.; van Bruggen, A.H.C.; Widmer, F.; Wyss, G.S.; Zijlstra, C.A.; Sessitsch, A.. |
| PathOrganic assesses risks associated with the consumption of fresh and minimally processed vegetables due to the prevalence of bacterial human pathogens in plant produce. The project evaluates whether organic production poses a risk on food safety, taking into consideration sources of pathogen transmission (e.g. animal manure). The project also explores whether organic versus conventional production practices may reduce the risk of pathogen manifestation. In Europe, vegetable-linked outbreaks are not well investigated. A conceptual model together with novel sampling strategies and specifically adjusted methods provides the basis for large-scale surveys of organically grown plant produce in five European countries. Critical control points are... |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: "Organics" in general; Vegetables. |
| Ano: 2009 |
URL: http://orgprints.org/14310/1/Arthurson_14310.pdf |
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| Forslund, A.; Ensink, J.; Battilani, A.; Kljujev, I.; Gola, S.; Raicevic, V.; Jovanovic, Z.; Stikic, R.; Sandei, L.; Fletcher, T.; Dalsgaard, A.. |
| Clean water has become one of the main limiting factors in agricultural food production in Europe, especially for countries around the Mediterranean, who now face more severe and frequent seasonal water shortages. In order to overcome water shortages the European Water Framework Directive encourages and promotes the use of treated urban wastewater in agriculture. However, the use of poor quality water in agriculture poses potential health risks. The application of wastewater through subsurface drip irrigation lines could possibly overcome public health concerns by minimizing contact with wastewater by farmers, farm workers but it is uncertain if the risk for consumers of wastewater irrigated produces would be acceptable. The objective of the current study... |
| Tipo: Journal paper |
Palavras-chave: Food security; Food quality and human health Irrigation and drainage. |
| Ano: 2010 |
URL: http://orgprints.org/18741/4/18741.pdf |
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| Jensen, A.N.; Dalsgaard, A.; Stockmarr, A.; Nielsen, E.M.; Baggesen, D.L.. |
| It was investigated how organic rearing conditions influence the Salmonella enterica infection dynamics in pigs and whether Salmonella persists in the paddock environment. Pigs inoculated with S. enterica serovar Typhimurium were grouped with Salmonella-negative tracer pigs. Bacteriological and serological testing indicated that organic pigs were susceptible to Salmonella infections, as 26 of 46 (56%) tracer pigs turned culture positive. An intermittent and mainly low-level excretion of Salmonella (<100 cells per g partly explains why the bacteriological prevalence appeared lower than the seroprevalence. Salmonella persisted in the paddock environment, as Salmonella was isolated from 46% of soil and water samples (n = 294). After removal of pigs,... |
| Tipo: Journal paper |
Palavras-chave: Food security; Food quality and human health Pigs. |
| Ano: 2006 |
URL: http://orgprints.org/9158/1/9158.pdf |
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| Registros recuperados: 10 | |
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