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Methodology for Single-Cell Genetic Analysis of Planktonic Foraminifera for Studies of Protist Diversity and Evolution ArchiMer
Weiner, Agnes K. M.; Morard, Raphael; Weinkauf, Manuel F. G.; Darling, Kate F.; André, Aurore; Quillévéré, Frédéric; Ujiie, Yurika; Douady, Christophe J.; De Vargas, Colomban; Kucera, Michal.
Single-cell genetic analysis is an essential method to investigate the biodiversity and evolutionary ecology of marine protists. In protist groups that do not reproduce under laboratory conditions, this approach provides the only means to directly associate molecular sequences with cell morphology. The resulting unambiguous taxonomic identification of the DNA sequences is a prerequisite for barcoding and analyses of environmental metagenomic data. Extensive single-cell genetic studies have been carried out on planktonic foraminifera over the past 20 years to elucidate their phylogeny, cryptic diversity, biogeography, and the relationship between genetic and morphological variability. In the course of these investigations, it has become evident that genetic...
Tipo: Text Palavras-chave: Foraminifera; Protists; Single-cell genetics; Cryptic diversity; Comparability; Methods; Laboratory protocols; Standardization.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00383/49460/49938.pdf
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Rapid switches in subpolar North Atlantic hydrography and climate during the Last Interglacial (MIS 5e) ArchiMer
Irvali, Nil; Ninnemann, Ulysses S.; Galaasen, Eirik V.; Rosenthal, Yair; Kroon, Dick; Oppo, Delia W.; Kleiven, Helga F.; Darling, Kate F.; Kissel, Catherine.
At the peak of the previous interglacial period, North Atlantic and subpolar climate shared many features in common with projections of our future climate, including warmer-than-present conditions and a diminished Greenland Ice Sheet (GIS). Here we portray changes in North Atlantic hydrography linked with Greenland climate during Marine Isotope Stage (MIS) 5e using (sub)centennially sampled records of planktonic foraminiferal isotopes and assemblage counts and ice-rafted debris counts, as well as modern analog technique and Mg/Ca-based paleothermometry. We use the core MD03-2664 recovered from a high accumulation rate site (similar to 34 cm/kyr) on the Eirik sediment drift (57 degrees 26.34'N, 48 degrees 36.35'W). The results indicate that surface waters...
Tipo: Text Palavras-chave: Eirik Drift; MIS 5e; North Atlantic; Last interglacial; Multiproxy.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00139/25046/23154.pdf
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Nomenclature for the Nameless: A Proposal for an Integrative Molecular Taxonomy of Cryptic Diversity Exemplified by Planktonic Foraminifera ArchiMer
Morard, Raphael; Escarguel, Gilles; Weiner, Agnes K. M.; Andre, Aurore; Douady, Christophe J.; Wade, Christopher M.; Darling, Kate F.; Ujiie, Yurika; Seears, Heidi A.; Quillevere, Frederic; De Garidel-thoron, Thibault; De Vargas, Colomban; Kucera, Michal.
Investigations of biodiversity, biogeography, and ecological processes rely on the identification of "species" as biologically significant, natural units of evolution. In this context, morphotaxonomy only provides an adequate level of resolution if reproductive isolation matches morphological divergence. In many groups of organisms, morphologically defined species often disguise considerable genetic diversity, which may be indicative of the existence of cryptic species. The diversity hidden by morphological species can be disentangled through genetic surveys, which also provide access to data on the ecological distribution of genetically circumscribed units. These units can be identified by unique DNA sequence motifs and allow studies of evolutionary and...
Tipo: Text Palavras-chave: Cryptic species; Genetic diversity; Planktonic foraminifera; Molecular nomenclature; MOTUs.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00383/49408/49900.pdf
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