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| Borzym, E.; Matras, M.; Maj-paluch, J.; Baud, M.; De Boisseson, C.; Talbi, Chiraz; Olesen, N. J.; Bigarre, L.. |
| A rhabdovirus was isolated in cell culture inoculated with tissue material from diseased grayling, Thymallus thymallus (L.), originating from a fish farm affected by a mortality episode in Poland. Diagnostics tests showed that the virus was not related to novirhabdoviruses known in Europe, nor to vesiculovirus-like species, except perch rhabdovirus (PRhV) with which it shared moderate serological relations. However, RT-PCR with PRhV probes gave negative results. To identify the virus, a random-priming sequence-independent single primer amplification was adopted. Surprisingly, two of the obtained sequences exhibited a high identity (>99%) with hirame rhabdovirus (HIRRV), a novirhabdovirus usually found in fish in marine Asiatic countries, for instance... |
| Tipo: Text |
Palavras-chave: Outbreak; Brown trout; Rhabdovirus; Sequence-independent single primer amplification; Molecular tracing; Grayling. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00197/30852/32727.pdf |
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