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| Silva,Francielle Gibson da; Freitas,Julio Cesar de; Anzai,Eleine Kuroki; Hashimoto,Vanessa Yumi; Giraldi,Nilson; Delbem,Ádina Cléia B.; Bracarense,Ana Paula F.R.L.; Reis,Antônio Carlos F. dos; Vasconcellos,Silvio Arruda. |
| The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14), from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct examination detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospire; Indirect immunoperoxidase; Immunohistochemistry; Cows; Culture. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100008 |
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| Soto,Francisco Rafael M.; Azevedo,Sérgio Santos de; Morais,Zenaide Maria de; Pinheiro,Sônia Regina; Delbem,Ádina Cléia B.; Moreno,Andréa Micke; Paixão,Renata; Vuaden,Erlete R.; Vasconcellos,Sílvio Arruda. |
| Leptospirosis is an important zoonosis that causes reproductive disorders in swine. The isolation of leptospires from aborted fetuses, stillbirths and weak piglets was obtained in several occasions, however, the bacteria was never isolated from apparently healthy piglets born from apparently healthy infected dams. Six sows of the Landrace breed with a known date of service and pregnancy confirmed by ultrasonography were infected intravenously with 5ml of Leptospira interrogans serovar Canicola inoculum between 76 and 90 days of gestation, and one week after farrowing, at least one piglet per sow was euthanized and samples of liver, kidneys, lungs, heart, spleen and gastric content were taken and examined by PCR. Reproductive disorders or any clinical sign... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Experimental swine leptospirosis; Leptospira spp.; Serovar Canicola; Vertical transmission. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400034 |
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