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Delbem,Ádina Cléia Botazzo; Freitas,Julio Cesar de; Bracarense,Ana Paula F. R. L.; Müller,Ernst Eckehardt; Oliveira,Rosângela Claret de. |
Swine leptospirosis is recognized world wide as an important cause of reproductive failure. Serum and histo-pathological examinations have been constantly used to diagnose this disease. This study was carried out on slaughtered sows from Northern Paraná State to compare serum findings with kidney, liver, ovary and uterus histo-pathological examinations. The microscopic serum-agglutination carried out on 36 animals detected 24 with titles > 100 and 12 negative (< 100). The histopathological examinations made on livers, kidneys, ovaries and uterus stained with hematoxylin-eosin, were carried out on 16 randomly chosen animals, 11 from the groups with > 100 serological titles and five from the negative group. The results showed that all the 16 animals... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Swine; Diagnostic. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200016 |
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Freitas,Julio Cesar de; Silva,Francielle Gibson da; Oliveira,Rosângela Claret de; Delbem,Ádina Cléia Botazzo; Müller,Ernst Eckehardt; Alves,Lucimara Aparecida; Teles,Paulo Sergio. |
Leptospira isolation allows definitive diagnosis of the infection. Contamination by microorganisms is one of the inconveniences of the culture. The objective of this study was to describe the isolation of Leptospira from dogs, bovine and swine naturally infected. Urine samples from 14 dogs and three bovines, and kidney, liver, ovary, and uterus body samples from 36 slaughtered sows with unknown health history, were used. The urine and organ samples were cultured in culture medium. Modified Ellinghausen-McCullough-Johnson-Harris medium (EMJH) culture medium was used with addition of 5-fluorouracil, chloramphenicol, vancomycin, nalidixic acid and neomycin. Incubation was performed at 28oC for 24 hours, followed by subculture in modified EMJH without... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Diagnostic; Culture; Cattle; Dog; Swine. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300030 |
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Delbem,Ádina Cléia Botazzo; Freire,Roberta Lemos; Silva,Caio Abércio da; Müller,Ernst Eckehardt; Dias,Ricardo Augusto; Ferreira Neto,José Soares; Freitas,Julio Cesar de. |
A leptospirose é uma zoonose amplamente difundida e de grande importância sócio-econômica para a suinocultura. Com o objetivo de individualizar condutas de manejo ou condições ambientais que estejam relacionadas ao risco de infecção por leptospirose, foram estudadas 298 matrizes suínas provenientes de granjas da região norte do Estado do Paraná. Todos os animais foram submetidos à prova de soroaglutinação microscópica e para cada um deles foi aplicado um questionário epidemiológico, que procurou investigar variáveis que poderiam estar associadas a essa infecção. Os resultados obtidos foram submetidos ao teste c², seguido de análise multivariada. Foram detectados títulos de anticorpos >100 em 132 matrizes suínas (44,3%), sendo em maior freqüência contra... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirose; Matrizes suínas; Fatores de risco; Diagnóstico. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300029 |
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