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DYNAPSE - DYNamiques des efflorescences et de la toxicité des espèces phytoplanctoniques nuisibles du genre PSEudo nitzschia en région Loire Bretagne ArchiMer
Belin, Catherine; Chapelle, Annie; Delmas, Daniel; Nezan, Elisabeth; Siano, Raffaele.
Les efflorescences de la diatomée Pseudo-nitzschia sont largement répandues sur les côtes Manche-Atlantique depuis une trentaine d’années (source Rephy). Depuis les années 2000 elles y sont parfois associées des événements toxiques du type ASP (Amnesic Shellfish Poisonning) qui perturbent grandement les pêcheries de coquillages et notamment celles de coquilles Saint Jacques. Les efflorescences et toxicités liées aux Pseudo-nitzschia sont des phénomènes en augmentation sur les côtes françaises mais aussi mondialement. Le genre Pseudo-nitzschia comprend plusieurs espèces très difficiles à identifier en routine or certaines sont toxiques et d’autres pas. Les facteurs environnementaux abiotiques et biotiques contrôlent à la fois la croissance des microalgues...
Tipo: Text Palavras-chave: Algues toxiques; Pseudo nitzschia; Toxicité des coquillages; Pêcheries; Facteurs environnementaux.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00189/30035/28521.pdf
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Les glucides et l'évolution de la matière organique dans les sédiments marins ArchiMer
Delmas, Daniel.
Carbohydrates have been analysed in arder to establish the state of organic matter in sediment. Samples were collected bath in neritic and oceanic (Orgon croises) areas. Reported data demonstrate that the insoluble-carbohydratesfsoluble-carbohydrates ratio (1/S) is a good indicator of organic matter evolution. In sediments, 1/S values ranging from 6 to 8 are associated with slightly-degraded organic matter, whereas values higher than 10 and eyen up to 30 relate to highly-degraded material. Furthermore, 1/S and CjN ratios generally show the same profile in sediments. The balance betweeh soluble (S) and insoluble (1) carbohydrates depends on the early stages of organic matter evolution, the soluble fraction being more easily degraded; such factors as...
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Ano: 1983 URL: http://archimer.ifremer.fr/doc/00120/23143/20988.pdf
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Premières propositions pour un indice de composition du phytoplancton, basé sur les résultats des méthodes pigments et diversité génétique. Action 3 - Indice Composition - Livrable 1. Rapport final, mars 2015. ArchiMer
Siano, Raffaele; Delmas, Daniel; Lampert, Luis.
Tipo: Text Palavras-chave: Phytoplancton; Pigments; Indice composition; Diversité pigmentaire; Metabarcoding; Picoplancton; Mamiellophyceae; Chlb; Golfe de Gascogne; Panache de la Gironde; Phytoplankton; Pigments; Composition index; Pigment diversity; Metabarcoding; Picoplankton; Mamiellophyceae; Chlb; Gulf of Biscay; River Gironde plume.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00361/47263/47228.pdf
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Exoproteolytic activity in an Atlantic pond (France): estimates of in situ activity ArchiMer
Crottereau, C; Delmas, Daniel.
In order to estimate in situ bacterial exoproteolysis and its contribution to bacterial secondary production (BSP), we studied microbial activities (BSP and kinetics of exoproteolytic activity) in relation to dissolved combined amino acids (DCAA) during the survey of an Atlantic marine pond. In this pond, high concentrations of DCAA (mean = 4.27 mu M), probably generated by zooplankton grazing, allowed large bacterial biomass development and high production (max = 10(11) cells l(-1) and 18.8 x 10(9) cells l(-1) d(-1) respectively). Exoproteolytic enzyme activity (mean V-m = 55.9 mu M d(-1)) was tightly cou pled to bacterial production, suggesting that protein utilization was essential for bacterial growth. Calculation, according to the Michaelis-Menten...
Tipo: Text Palavras-chave: Bacterioplankton; In situ exoproteolytic hydrolysis rate; Kinetic parameters; DCAA; Marine pond.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00446/55803/62288.pdf
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Selective liquid chromatographic determination of trace domoic acid in seawater and phytoplankton: improvement using the o-phthaldialdehyde/9-fluorenylmethylchloroformate derivatization ArchiMer
Devez, Aurelie; Delmas, Daniel.
Domoic acid (DA), a toxin produced worldwide by some species of the genus Pseudo-nitzschia, is responsible for contamination of marine molluscs, mammals, birds, and for human intoxication, and when detected in high levels results in closures of shellfish farms, thus causing severe economic losses to aquaculture. Studies on algal production of DA in cultures and field samples require sensitive methods capable of measuring trace concentrations of domoic acid. Measuring domoic acid concentrations at trace levels is still a significant challenge. A sensitive and reliable double derivatization using o-phthaldialdehyde-mercaptoethanol/9-fluorenylmethylchloroformate (OPA-MeSH/FMOC-Cl) followed by high-performance liquid chromatography with fluorescence detection...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00151/26234/24438.pdf
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Lower trophic levels and detrital biomass control the Bay of Biscay continental shelf food web: Implications for ecosystem management ArchiMer
Lassalle, G.; Lobry, J.; Le Loc'H, F.; Bustamante, Paco; Certain, Gregoire; Delmas, Daniel; Dupuy, C.; Hily, Christian; Labry, Claire; Le Pape, O.; Marquis, Elise; Petitgas, Pierre; Pusineri, Claire; Ridoux, Vincent; Spitz, J.; Niquil, Nathalie.
The Bay of Biscay (North-East Atlantic) has long been subjected to intense direct and indirect human activities that lead to the excessive degradation and sometimes overexploitation of natural resources. Fisheries management is gradually moving away from single-species assessments to more holistic, multi-species approaches that better respond to the reality of ecosystem processes. Quantitative modelling methods such as Ecopath with Ecosim can be useful tools for planning, implementing and evaluating ecosystem-based fisheries management strategies. The aim of this study was therefore to model the energy fluxes within the food web of this highly pressured ecosystem and to extract practical information required in the diagnosis of ecosystem state/health. A...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00057/16851/14329.pdf
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Définition d’un indice composition pour le phytoplancton en Manche- Atlantique, à partir des données du micro-phytoplancton du REPHY et des réseaux régionaux, et des mesures complémentaires de la flore phytoplanctonique acquises avec des techniques novatrices. Action 3 – Livrable 1. Propositions pour un indice de composition du phytoplancton, basé sur les résultats des méthodes microscopie, pigments et diversité génétique. Rapport final, février 2016 ArchiMer
Hernandez Farinas, Tania; Brun, Melanie; Siano, Raffaele; Delmas, Daniel.
This deliverable is composed of two reports which propose indice of phytoplankton composition, based respectively on the results of pigment and genetic diversity methods, and the results of microscopy method. Proposals based on the results of pigment and genetic diversity methods. The present work is a continuation of several studies carried out in the past (Siano and Delmas, 2013, 2015) on investigating an indicator of phytoplankton diversity using two methods complementary to optical microscopy: the genetic diversity analyzed with metabarcoding (exhaustive analysis of the diversity and relative abundance of OTUs, i.e. groups of sequences identified by their genetic resemblance) and the pigmentary diversity (analysis of the diversity and abundance of...
Tipo: Text Palavras-chave: Metabarcoding; OTUs; Pigments; Picophytoplancton; Indice composition; Niche ecologique; Mamiellophyceae; Chlorophylle b; Rade de Brest; Bretagne sud; Panache de la Gironde; Denombrements du micro-phytoplancton; Abondance totale; Monotonicite; Linearite; Pressions; Facade Manche-Atlantique; Metabarcoding; OTUs; Pigments; Picophytoplankton; Composition indice; Ecological niche; Mamiellophyceae; Chlorophyll b; Brest Bay; Southern Britain; Gironde plume; Micro-phytoplankton counts; Total abundance; Monotonicity; Linearity; Pressure; Channel-Atlantic coast.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00363/47421/47426.pdf
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Exoproteolytic activity determined by flow injection analysis: its potential importance for bacterial growth in coastal marine ponds ArchiMer
Delmas, Daniel; Legrand, Catherine; Bechemin, Christian; Collinot, Cecile.
The measurement of the fluorescent 4-methyl-7-coumarinylamine released from the hydrolysis of a non-fluorescent peptide model substrate by exoproteolytic enzymes has been adapted to flow injection analysis (FIA). FIA allows samples to be processed very quickly (less than 2 min. for triplicate determinations) with good sensitivity (< 0.1 muM) and reproducibility (relative standard deviation < 3 % at the 0.2 muM level). In a coastal marine pond, exoproteolytic activity was closely related to bacterioplankton biomass. The high activity measured in pond water (maximum velocity: V(M) almost-equal-to 1.46 to 2.54 muM.h-1) emphasizes the importance of dissolved protein hydrolysis for bacterial growth and for dissolved organic nitrogen cycling. The mean...
Tipo: Text Palavras-chave: Activité exoprotéolytique; Protéine dissoute; Analyse en flux continu; Bactérioplancton; Marais maritime; Flow injection analysis; Exoproteolytic activity; Dissolved protein; Bacterioplankton; Coastal marine pond.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00196/30756/29163.pdf
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Stimulation of bacterial exoproteolytic activity by fish farming in coastal marine ponds: Effect on dissolved protein cycling ArchiMer
Garet, Marie-josé; Reymond, Hugues; Delmas, Daniel.
The effect of fish farming on dissolved amino acid concentrations, bacterioplankton abundance and exoproteolytic activity was assessed in 3 experimental marine ponds. Different standing stocks of fish were introduced (semi-intensive pond: 250 g.m(-2); semi-extensive pond: 50 g.m(-2) control pond: 0). Sea bass farming increased dissolved combined amino acid (DCAA) concentrations only in the semi-intensive pond. Bacterial standing stock was unaffected by fish food supply. However, bacterial exoproteolytic activity was strongly stimulated by aquaculture intensification; the average maximal rate of dissolved protein hydrolysis (V-m) increased with intensity (control pond: 1 500 nM.h(-1); semi-extensive pond: 2 600 nM.h(-1) semi-intensive pond: 5 100 nM.h(-1))....
Tipo: Text Palavras-chave: Activité exoprotéolytique bactérienne; Protéines dissoutes; Intensification de l'aquaculture; Marais maritimes; Bacterial exoproteolytic activity; Dissolved proteins; Aquaculture; Coastal marine ponds.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00336/44766/44701.pdf
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Phytoplankton and bacterial alkaline phosphatase activities in relation to phosphate and DOP availability within the Gironde plume waters (Bay of Biscay) ArchiMer
Labry, Claire; Delmas, Daniel; Herbland, Alain.
Previous studies conducted on the continental shelf in the Southeast Bay of Biscay influenced by Gironde waters (one of the two largest rivers on the French Atlantic coast) showed the occurrence of late winter phytoplankton blooms and phosphorus limitation of algal growth thereafter. In this context, the importance of dissolved organic phosphorus (DOP) for both algae and bacteria was investigated in 1998 and 1999 in terms of stocks and fluxes. Within the mixed layer, although phosphate decreased until exhaustion from winter to spring, DOP remained high and phosphate monoesters made up between I I to 65% of this pool. Total alkaline phosphatase activity (APA, V-max) rose gradually from winter (2-8 nM h(-1)) to late spring (100-400 nM h-1), which was mainly...
Tipo: Text Palavras-chave: Phytoplankton; Phosphate; Gironde; DOP; Bacteria; Alkaline phosphatases.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-608.pdf
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Determining how the pelagic ecosystem over the continental shelf of the Bay of Biscay (NE Atlantic) functions: An approach using mesozooplankton enzyme activities as descriptors ArchiMer
Bergeron, Jean-pierre; Delmas, Daniel; Koueta, Noussithe.
A fisheries research cruise conducted in 2000 offered a first opportunity to take simultaneous measurements of the activities of three enzymes in mesozooplankton samples collected at a regional scale over the continental shelf of the Bay of Biscay in the NE Atlantic, with the aim of characterizing main aspects of the functioning of the biotic environment of small pelagic fish populations. The activity of the digestive endopeptidase trypsin was selected to characterize the assimilation rate of proteins, whereas pyruvate kinase (PK) was chosen as an indicator of carbohydrate assimilation and aspartate transcarbamylase (ATC) provided an overall assessment of mesozooplankton productivity. The Bay of Biscay region is subject to various strong physical driving...
Tipo: Text Palavras-chave: Trypsin; Pyruvate kinase; Metabolic descriptors; Mesozooplankton; Bay of Biscay; Aspartate transcarbamylase.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6359.pdf
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Acquisition de données complémentaires aux dénombrements, avec les techniques d’analyses pigmentaires et de diversité génétique. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° AIV. Rapport final, 16 juillet 2014. ArchiMer
Delmas, Daniel; Siano, Raffaele.
Studies on phytoplankton community diversity cannot be limited microphytoplankton (cells>20 ìm), it is indeed necessary to take into account also the nano (3-20ìm) picophytoplankton (<3 ìm) components. In the frame of this work aimed at studying temporal phytoplankton community diversity two complementary methodologies of phytoplankton sample analysis, pigment and genetic (metarbarcoding) diversity, have been used as alternative to routine morphological analysis carried out at light microscopy. The REPHY station (047-P-016 Concarneau Large) off-shore the Concarneau Bay has been sampled during the annual highest phytoplankton production (March-July 2012) with a frequent sample interval strategy (twice per week). Results obtained by both pigment and...
Tipo: Text Palavras-chave: Phytoplancton; Structure de taille; Picophytoplancton; Nanophytoplancton; Microphytoplancton; Diversité génétique; Metabarcoding; Taxonomie pigmentaire; Façade atlantique; Baie de Concarneau; Phytoplankton; Size classes; Picophytoplankton; Nanophytoplankton; Microphytoplankton; Genetic diversity; Metabarcoding; Pigment taxonomy; Atlantic coasts; Concarneau Bay.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47260/47225.pdf
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Conditions d'apparition de densités accrues de dinoflagellés Dinophysis dans le pertuis d'Antioche et les eaux adjacentes. Campagnes Dinopertuis 89 ArchiMer
Delmas, Daniel; Herbland, Alain; Maestrini, Serge; Mornet, Francoise; Morand, Philippe; Spano, Anna-maria; Burgeot, Thierry; Margat, Sylvie; Charpentier, Gabriel; Fillon, Alain; Leguay, Didier; Thomas, Gerard; Bacher, Cedric; Heral, Maurice; Prou, Jean.
Environmental and species features which lead to increases in cell density of the D.S.P. Dinoflagellate Dinophysis in inshore and offshore waters of the île de Ré - île d'Oléron area, France (cruises "Dinopertuis89"). Four weekly sampling series have been carried out, within 10 May – 31 May 1989, at 24 stations located from inshore to offshore waters of the "île de Ré" - "île d’Oléron" area, with the aim to investigate (i) whether Dinophysis increases in cell density occur first in offshore waters and then, eventually, are pushed to the coast, or develop simultaneously in the whole area, and (ii) whether there are some environmental conditions which are related to Dinophysis blooms. Depths of sampling have been stated on the bas is of STD profiles. All...
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Ano: 1990 URL: http://archimer.ifremer.fr/doc/00174/28532/26916.pdf
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Satellite-derived parameters for biological modelling in coastal waters: Illustration over the eastern continental shelf of the Bay of Biscay ArchiMer
Gohin, Francis; Loyer, S; Lunven, Michel; Labry, Claire; Froidefond, J; Delmas, Daniel; Huret, Martin; Herbland, Alain.
In biological modelling of the coastal phytoplankton dynamics, the light attenuation coefficient is often expressed as a function of the concentrations of chlorophyll and mineral suspended particulate matter (SPM). In order to estimate the relationship between these parameters over the continental shelf of the northern Bay of Biscay, a set of in situ data has been gathered for the period 1998-2003 when SeaWiFS imagery is available. These data comprise surface measurements of the concentrations of total SPM, chlorophyll, and irradiance profiles from which is derived the attenuation coefficient of the photosynthetically available radiation, K-PAR. The performance of the IFREMER look-up table used to retrieve the chlorophyll concentration from the SeaWiFS...
Tipo: Text Palavras-chave: Bay of Biscay; SeaWiFS; Sediment; Chlorophyll; Ocean color.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1172.pdf
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Minéralisation de la matière organique et échanges ioniques à l'interface eau-sédiment de l'étang de Berre, Méditerranée ArchiMer
Delmas, Daniel.
The étang de Berre, near Marseilles (France), is an inland brackish basin with a high primary rate of production. In 1966, the diversion of the Durance River introduced important hydrological changes. A study of organic matter mineralization and ionie exchanges at the water-sediment interface has made it possible to distinguish two areas, according to the composition of organic matter, mineralization mechanism and ionie transfers. In the North, the high rate of mineral sedimentation leads to a low content of organic matter in the sediment. This organic matter of detrital origin is little affected by mineralization; there is, however, high production of ammonium nitrogen, which is released to overlying waters. Nitrates and nitrites undergo denitrification...
Tipo: Text Palavras-chave: Interface eau-sédiment; Matière organique; Eau interstitielle; Minèralisation; Milieu saumâtre; Water-sediment interface; Organic matter; Lnterstitial water; Mineralization; Brackish basin.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/00122/23317/21142.pdf
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Environmental conditions which lead to increase in cell density of the toxic dinoflagellates Dinophysis spp. in nutrient-rich and nutrient-poor waters of the French Atlantic coast ArchiMer
Delmas, Daniel; Herbland, Alain; Maestrini, Serge.
From 22 April to 19 June 1990, 7 stations located off La Rochelle, France, from near-shore ('bassin Marennes-Oleron') to offshore waters (50 m depth contour) were visited 8 times, with the aim of determining whether the toxic dinoflagellates Dinophysis spp. increase in cell density in nutrient-poor offshore water, in nutrient-rich inshore waters, or both, and to determine their vertical distribution in relation to stratification. Temperature stratification developed offshore, but not inshore. In April, few Dinophysis spp. were present offshore and none inshore. In late May, up to 15 X 10(3) cells l(-1) were recorded in the 10 to 15 m layer offshore, whereas inshore waters contained only a few cells l(-1). In late May, a short period of wind partly modified...
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/00190/30141/28772.pdf
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La biodéposition dans les aires ostréicoles. Son rôle dans la concentration de la matière organique et de contaminants potentiels. Son impact sur le sédiment. ArchiMer
Martin, Jean-louis; Sornin, Jean-marc; Delmas, Daniel; El Sayed, Mohamed A.; Berthet, Brigitte.
Au cours de cette étude, nous avons étudié l'impact de la biodéposition sur les bassins conchylicoles. La biodéposition constitue un "concentrateur" de particules fines au niveau du sédiment. Cet impact est particulièrement évident sur les sédiments sableux et concerne la granulométrie, les taux de matière organique, la concentration des métaux et des hydrocarbures. Une étude comparée est faite entre estran sableux et bassin de type "claire" à sédiment argileux. L'impact de la biodéposition sur les sédiments sableux n'est pas irréversible. Le bassin fermé avec un sédiment argileux agit comme un concentrateur-rétenteur de particules et des contaminants potentiels. Ceci souligne le rôle "tampon" des marais maritimes entre milieu continental et milieu marin.
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Ano: 1989 URL: http://archimer.ifremer.fr/doc/00076/18757/16327.pdf
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Evolutions quantitatives et qualitatives du seston dans une claire à huîtres : relation avec la sédimentation et la biodéposition ArchiMer
Sornin, Jean-marc; Delmas, Daniel; Deslous-paoli, Jean-marc.
Dans un bassin aquacole fermé (claires à huîtres), l'évolution qualitative et quantitative des matières en suspension subit des variations saisonnières directement liées aux capacité de production planctonique. Ainsi, à la fin du printemps et en été, le réchauffement des eaux s'accompagne d'une augmentation de matière organique importante (jusqu'à 17 mg.1(-1). Pendant cette période, la sédimentation naturelle est supérieure à 350 g.m(-2).j(-1). La biodéposition (fèces + pseudofèces) suit l'évolution des capacités de rétention des huîtres. Dans ce type de bassin fermé, cette capacité est liée à la qualité des matières en suspension. En période estivale, les fortes teneurs en matière organique du seston (entre 5 et 17 mg.1(-1)) dispensent les mollusques...
Tipo: Text Palavras-chave: Crassostrea gigas; Oysters; Biodeposition; Sedimentation; Suspension; Particulate organic matter; Crassostrea gigas; Huîtres; Biodéposition; Sédimentation; Suspension; Matière organique particulaire.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/publication-7309.pdf
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Hydro-plankton characteristics and their relationship with sardine and anchovy distributions on the French shelf of the Bay of Biscay ArchiMer
Petitgas, Pierre; Masse, Jacques; Bourriau, Paul; Beillois, Pierre; Bergeron, Jean-pierre; Delmas, Daniel; Herbland, Alain; Koueta, Noussithe; Froidefond, Jean-marie; Santos, Maria.
The spatial pattern in hydro-plankton and fish distributions and their relationship were analysed based on the spring 2000 fisheries acoustic survey. The importance of this survey was that it was a multi-disciplinary platform which collected an extensive set of parameters in the hydro-plankton leading to a potentially finer description of hydro-plankton conditions and fish habitats. More than 50 variables were measured on a grid of stations, in four compartments of the ecosystem: hydrology, nutrients, primary producers and meso-zooplankton. First, a joint analysis of all hydro-plankton compartments was performed using multiple factor analysis (MFA). The method was used to estimate a compromise factorial space common to all compartments in which the...
Tipo: Text Palavras-chave: Fish environment relationship; Hydro plankton; Habitats; Multiple factor analysis; Spatial pattern.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-3961.pdf
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Addressing the measurement of particulate organic and inorganic phosphorus in estuarine and coastal waters ArchiMer
Labry, Claire; Youenou, Agnes; Delmas, Daniel; Michelon, Pascale.
The speciation of particulate phosphorus (TPP) into inorganic (PIP) and organic phosphorus (POP), has been scarcely investigated in suspended particulate matter (SPM), contrary to numerous sediment studies. This work evaluates the actual capacity of methods for TPP, POP and PIP determination in SPM to measure efficiently and specifically each pool. Methods were tested on a range of plankton-associated organic and inorganic P compounds, on microalgal cultures and estuarine samples. Then results are specifically discussed reviewing P speciation in phytoplankton. For TPP analysis, the method of Solorzano and Sharp (1980) is recommended compared with that of Aspila et al. (1976), originally dedicated to sediment, as there is one step less, the protocol is more...
Tipo: Text Palavras-chave: Phosphorus; Speciation; Waters; Phytoplankton; HTC; CWO.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00149/26046/26385.pdf
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