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Determining how the pelagic ecosystem over the continental shelf of the Bay of Biscay (NE Atlantic) functions: An approach using mesozooplankton enzyme activities as descriptors ArchiMer
Bergeron, Jean-pierre; Delmas, Daniel; Koueta, Noussithe.
A fisheries research cruise conducted in 2000 offered a first opportunity to take simultaneous measurements of the activities of three enzymes in mesozooplankton samples collected at a regional scale over the continental shelf of the Bay of Biscay in the NE Atlantic, with the aim of characterizing main aspects of the functioning of the biotic environment of small pelagic fish populations. The activity of the digestive endopeptidase trypsin was selected to characterize the assimilation rate of proteins, whereas pyruvate kinase (PK) was chosen as an indicator of carbohydrate assimilation and aspartate transcarbamylase (ATC) provided an overall assessment of mesozooplankton productivity. The Bay of Biscay region is subject to various strong physical driving...
Tipo: Text Palavras-chave: Trypsin; Pyruvate kinase; Metabolic descriptors; Mesozooplankton; Bay of Biscay; Aspartate transcarbamylase.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6359.pdf
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Microbial enzymatic assays in environmental water samples: impact of Inner Filter Effect and substrate concentrations ArchiMer
Urvoy, Marion; Labry, Claire; Delmas, Daniel; Creac'H, Layla; L Helguen, Stephane.
As microbial enzymatic activities initiate the mineralization of organic matter through the microbial loop, it is important to correctly measure those activities and be able to perform inter-study comparisons. Enzymatic activity assays are typically carried out using fluorogenic substrate analogs, such as 4-Methylumbelliferone and 7-Amino-4-methylcoumarin linked to sugar monomers, phosphate group or amino acids. However, methodological divergences can be found in aquatic science literature, potentially leading to misestimated activities. To highlight some of those methodological key points, we first addressed the potential occurrence of an Inner Filter Effect (IFE), a fluorometric artifact that affects the relationship between fluorophore concentration and...
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00653/76524/77614.pdf
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La biodéposition dans les aires ostréicoles. Son rôle dans la concentration de la matière organique et de contaminants potentiels. Son impact sur le sédiment. ArchiMer
Martin, Jean-louis; Sornin, Jean-marc; Delmas, Daniel; El Sayed, Mohamed A.; Berthet, Brigitte.
Au cours de cette étude, nous avons étudié l'impact de la biodéposition sur les bassins conchylicoles. La biodéposition constitue un "concentrateur" de particules fines au niveau du sédiment. Cet impact est particulièrement évident sur les sédiments sableux et concerne la granulométrie, les taux de matière organique, la concentration des métaux et des hydrocarbures. Une étude comparée est faite entre estran sableux et bassin de type "claire" à sédiment argileux. L'impact de la biodéposition sur les sédiments sableux n'est pas irréversible. Le bassin fermé avec un sédiment argileux agit comme un concentrateur-rétenteur de particules et des contaminants potentiels. Ceci souligne le rôle "tampon" des marais maritimes entre milieu continental et milieu marin.
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Ano: 1989 URL: http://archimer.ifremer.fr/doc/00076/18757/16327.pdf
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DYNAPSE - DYNamiques des efflorescences et de la toxicité des espèces phytoplanctoniques nuisibles du genre PSEudo nitzschia en région Loire Bretagne ArchiMer
Belin, Catherine; Chapelle, Annie; Delmas, Daniel; Nezan, Elisabeth; Siano, Raffaele.
Les efflorescences de la diatomée Pseudo-nitzschia sont largement répandues sur les côtes Manche-Atlantique depuis une trentaine d’années (source Rephy). Depuis les années 2000 elles y sont parfois associées des événements toxiques du type ASP (Amnesic Shellfish Poisonning) qui perturbent grandement les pêcheries de coquillages et notamment celles de coquilles Saint Jacques. Les efflorescences et toxicités liées aux Pseudo-nitzschia sont des phénomènes en augmentation sur les côtes françaises mais aussi mondialement. Le genre Pseudo-nitzschia comprend plusieurs espèces très difficiles à identifier en routine or certaines sont toxiques et d’autres pas. Les facteurs environnementaux abiotiques et biotiques contrôlent à la fois la croissance des microalgues...
Tipo: Text Palavras-chave: Algues toxiques; Pseudo nitzschia; Toxicité des coquillages; Pêcheries; Facteurs environnementaux.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00189/30035/28521.pdf
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Premières propositions pour un indice de composition du phytoplancton, basé sur les résultats des méthodes pigments et diversité génétique. Action 3 - Indice Composition - Livrable 1. Rapport final, mars 2015. ArchiMer
Siano, Raffaele; Delmas, Daniel; Lampert, Luis.
Tipo: Text Palavras-chave: Phytoplancton; Pigments; Indice composition; Diversité pigmentaire; Metabarcoding; Picoplancton; Mamiellophyceae; Chlb; Golfe de Gascogne; Panache de la Gironde; Phytoplankton; Pigments; Composition index; Pigment diversity; Metabarcoding; Picoplankton; Mamiellophyceae; Chlb; Gulf of Biscay; River Gironde plume.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00361/47263/47228.pdf
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Conditions d'apparition de densités accrues de dinoflagellés Dinophysis dans le pertuis d'Antioche et les eaux adjacentes. Campagnes Dinopertuis 89 ArchiMer
Delmas, Daniel; Herbland, Alain; Maestrini, Serge; Mornet, Francoise; Morand, Philippe; Spano, Anna-maria; Burgeot, Thierry; Margat, Sylvie; Charpentier, Gabriel; Fillon, Alain; Leguay, Didier; Thomas, Gerard; Bacher, Cedric; Heral, Maurice; Prou, Jean.
Environmental and species features which lead to increases in cell density of the D.S.P. Dinoflagellate Dinophysis in inshore and offshore waters of the île de Ré - île d'Oléron area, France (cruises "Dinopertuis89"). Four weekly sampling series have been carried out, within 10 May – 31 May 1989, at 24 stations located from inshore to offshore waters of the "île de Ré" - "île d’Oléron" area, with the aim to investigate (i) whether Dinophysis increases in cell density occur first in offshore waters and then, eventually, are pushed to the coast, or develop simultaneously in the whole area, and (ii) whether there are some environmental conditions which are related to Dinophysis blooms. Depths of sampling have been stated on the bas is of STD profiles. All...
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Ano: 1990 URL: http://archimer.ifremer.fr/doc/00174/28532/26916.pdf
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Stimulation of bacterial exoproteolytic activity by fish farming in coastal marine ponds: Effect on dissolved protein cycling ArchiMer
Garet, Marie-josé; Reymond, Hugues; Delmas, Daniel.
The effect of fish farming on dissolved amino acid concentrations, bacterioplankton abundance and exoproteolytic activity was assessed in 3 experimental marine ponds. Different standing stocks of fish were introduced (semi-intensive pond: 250 g.m(-2); semi-extensive pond: 50 g.m(-2) control pond: 0). Sea bass farming increased dissolved combined amino acid (DCAA) concentrations only in the semi-intensive pond. Bacterial standing stock was unaffected by fish food supply. However, bacterial exoproteolytic activity was strongly stimulated by aquaculture intensification; the average maximal rate of dissolved protein hydrolysis (V-m) increased with intensity (control pond: 1 500 nM.h(-1); semi-extensive pond: 2 600 nM.h(-1) semi-intensive pond: 5 100 nM.h(-1))....
Tipo: Text Palavras-chave: Activité exoprotéolytique bactérienne; Protéines dissoutes; Intensification de l'aquaculture; Marais maritimes; Bacterial exoproteolytic activity; Dissolved proteins; Aquaculture; Coastal marine ponds.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00336/44766/44701.pdf
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Evolutions quantitatives et qualitatives du seston dans une claire à huîtres : relation avec la sédimentation et la biodéposition ArchiMer
Sornin, Jean-marc; Delmas, Daniel; Deslous-paoli, Jean-marc.
Dans un bassin aquacole fermé (claires à huîtres), l'évolution qualitative et quantitative des matières en suspension subit des variations saisonnières directement liées aux capacité de production planctonique. Ainsi, à la fin du printemps et en été, le réchauffement des eaux s'accompagne d'une augmentation de matière organique importante (jusqu'à 17 mg.1(-1). Pendant cette période, la sédimentation naturelle est supérieure à 350 g.m(-2).j(-1). La biodéposition (fèces + pseudofèces) suit l'évolution des capacités de rétention des huîtres. Dans ce type de bassin fermé, cette capacité est liée à la qualité des matières en suspension. En période estivale, les fortes teneurs en matière organique du seston (entre 5 et 17 mg.1(-1)) dispensent les mollusques...
Tipo: Text Palavras-chave: Crassostrea gigas; Oysters; Biodeposition; Sedimentation; Suspension; Particulate organic matter; Crassostrea gigas; Huîtres; Biodéposition; Sédimentation; Suspension; Matière organique particulaire.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/publication-7309.pdf
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Potential Effect of Freshwater Virus on the Structure and Activity of Bacterial Communities in the Marennes-Oléron Bay (France) ArchiMer
Auguet, Jean-christophe; Montanie, Helene; Hartmann, Hans; Lebaron, P.; Casamayor, E. O.; Catala, P.; Delmas, Daniel.
Batch culture experiments using viral enrichment were conducted to test the response of a coastal bacterial community to autochthonous (i.e., co-existing) or allochthonous riverine viruses. The effects of viral infections on bacterial dynamics and activity were assessed by epifluorescence microscopy and thymidine incorporation, respectively, whereas the effect of viral infection on bacterial community composition was examined by polymerase chain reaction-single strand conformation polymorphism 16S ribosomal RNA fingerprinting. The percentages of high nucleic acid-containing cells, evaluated by flow cytometry, were significantly correlated (r (2) = 0.91, n = 12, p < 0.0001) to bacterial production, making this value a good predictor of active cell...
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6636.pdf
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Lower trophic levels and detrital biomass control the Bay of Biscay continental shelf food web: Implications for ecosystem management ArchiMer
Lassalle, G.; Lobry, J.; Le Loc'H, F.; Bustamante, Paco; Certain, Gregoire; Delmas, Daniel; Dupuy, C.; Hily, Christian; Labry, Claire; Le Pape, O.; Marquis, Elise; Petitgas, Pierre; Pusineri, Claire; Ridoux, Vincent; Spitz, J.; Niquil, Nathalie.
The Bay of Biscay (North-East Atlantic) has long been subjected to intense direct and indirect human activities that lead to the excessive degradation and sometimes overexploitation of natural resources. Fisheries management is gradually moving away from single-species assessments to more holistic, multi-species approaches that better respond to the reality of ecosystem processes. Quantitative modelling methods such as Ecopath with Ecosim can be useful tools for planning, implementing and evaluating ecosystem-based fisheries management strategies. The aim of this study was therefore to model the energy fluxes within the food web of this highly pressured ecosystem and to extract practical information required in the diagnosis of ecosystem state/health. A...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00057/16851/14329.pdf
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Use of SeaWiFS data for light availability and parameter estimation of a phytoplankton production model of the Bay of Biscay ArchiMer
Huret, Martin; Gohin, Francis; Delmas, Daniel; Lunven, Michel; Garcon, Véronique.
Processing SeaWiFS (Sea-viewing Wide Field-of-view Sensor) data provides useful information for the observation and modelling of the phytoplankton production of the Bay of Biscay. Empirical algorithms allow the retrieval of chlorophyll a and non-living Suspended Particulate Matter (SPM) concentrations. These data are used to constrain a coupled 3D physical-biogeochemical model of the Bay of Biscay continental shelf Two issues are investigated, depending on the variable used, to constrain the winter to spring phytoplankton production for the year 2001. First, SPM data is used as forcing data to correct the corresponding state variable of our model. This allows the realistic simulation of the light limited bloom at the end of February 2001, as observed with...
Tipo: Text Palavras-chave: Bay of Biscay; SeaWiFS; Parameter estimation; Chlorophyll; Suspended Particulate Matter; Physical biogeochemical modelling.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2493.pdf
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Exoproteolytic activity in an Atlantic pond (France): estimates of in situ activity ArchiMer
Crottereau, C; Delmas, Daniel.
In order to estimate in situ bacterial exoproteolysis and its contribution to bacterial secondary production (BSP), we studied microbial activities (BSP and kinetics of exoproteolytic activity) in relation to dissolved combined amino acids (DCAA) during the survey of an Atlantic marine pond. In this pond, high concentrations of DCAA (mean = 4.27 mu M), probably generated by zooplankton grazing, allowed large bacterial biomass development and high production (max = 10(11) cells l(-1) and 18.8 x 10(9) cells l(-1) d(-1) respectively). Exoproteolytic enzyme activity (mean V-m = 55.9 mu M d(-1)) was tightly cou pled to bacterial production, suggesting that protein utilization was essential for bacterial growth. Calculation, according to the Michaelis-Menten...
Tipo: Text Palavras-chave: Bacterioplankton; In situ exoproteolytic hydrolysis rate; Kinetic parameters; DCAA; Marine pond.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00446/55803/62288.pdf
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Nitrogenous nutrient transfers in oyster ponds role of sediment in deferred primary production ArchiMer
Sornin, Jean-marc; Collos, Yves; Delmas, Daniel; Feuillet-girard, Michèle; Gouleau, Daniel.
The oyster pond under study has a natural sediment bottom and water is changed only once or twice every month depending on tidal height. Between successive water renewals, the pond is a closed system, equivalent to a batch culture in which sinking and biodeposition of particulate matter are the only sources of organic matter to the bottom. Particulate organic matter enrichment of the sediment in winter is followed by intensive ammonification in summer with very limited nitrification. Seasonal nitrogen budgets involving particulate and dissolved phases show that in summer during batch mode period, ammonium enrichment from the sediment to the overlying water is an order of magnitude higher than the initial nutrient supply in the feed water. This allows a...
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Ano: 1990 URL: http://archimer.ifremer.fr/doc/00178/28892/27749.pdf
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Acquisition de données complémentaires aux dénombrements, avec les techniques d’analyses pigmentaires et de diversité génétique. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° AIV. Rapport final, 16 juillet 2014. ArchiMer
Delmas, Daniel; Siano, Raffaele.
Studies on phytoplankton community diversity cannot be limited microphytoplankton (cells>20 ìm), it is indeed necessary to take into account also the nano (3-20ìm) picophytoplankton (<3 ìm) components. In the frame of this work aimed at studying temporal phytoplankton community diversity two complementary methodologies of phytoplankton sample analysis, pigment and genetic (metarbarcoding) diversity, have been used as alternative to routine morphological analysis carried out at light microscopy. The REPHY station (047-P-016 Concarneau Large) off-shore the Concarneau Bay has been sampled during the annual highest phytoplankton production (March-July 2012) with a frequent sample interval strategy (twice per week). Results obtained by both pigment and...
Tipo: Text Palavras-chave: Phytoplancton; Structure de taille; Picophytoplancton; Nanophytoplancton; Microphytoplancton; Diversité génétique; Metabarcoding; Taxonomie pigmentaire; Façade atlantique; Baie de Concarneau; Phytoplankton; Size classes; Picophytoplankton; Nanophytoplankton; Microphytoplankton; Genetic diversity; Metabarcoding; Pigment taxonomy; Atlantic coasts; Concarneau Bay.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47260/47225.pdf
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Initiation of winter phytoplankton blooms within the Gironde plume waters in the Bay of Biscay ArchiMer
Labry, Claire; Herbland, Alain; Delmas, Daniel; Laborde, P; Lazure, Pascal; Froidefond, J; Jegou, Anne-marie; Sautour, B.
Thermostratification and seasonal Light increase are generally considered the first causes of phytoplankton spring blooms in temperate waters. The objective of this study is to confirm the existence of winter phytoplankton blooms, responsible for the early exhaustion of phosphate, within the Gironde plume waters (southeast Bay of Biscay), and to understand what may initiate them so early. Two cruises, BIOMET 2 and BIOMET 3, were carried out respectively in early (8 to 21 January) and late winter 1998 (25 February to 11 March). An increase of phytoplankton biomass (chl a) between the 2 cruises and non-conservative nutrients observed in late winter confirm an early phytoplankton growth both in the Gironde plume and adjacent oceanic waters. Among factors that...
Tipo: Text Palavras-chave: Vertical attenuation coefficient; Halostratification; Light availability; Winter blooms.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-810.pdf
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Do river discharge rates drive the overall functioning of the pelagic ecosystem over the continental shelf of the Bay of Biscay (NE Atlantic)? A comparison of two contrasting years with special reference to anchovy (Engraulis encrasicolus L.) nutritional state ArchiMer
Bergeron, Jean-pierre; Delmas, Daniel; Koueta, Noussithe.
In the 2000 and 2001 springtime periods, mesozooplankton samples were systematically taken during research cruises devoted to the study of the distribution and abundance of small pelagic fishes in the French part of the Bay of Biscay, NE Atlantic, continental shelf. Simultaneous activities of three enzymes were measured in these mesozooplankton samples, to characterize the main features of ecosystem function at a regional scale. The aim was to assess assimilation rates of carbohydrates, through pyruvate kinase activity, and protein, through trypsin activity, by the mesozooplankton community and the resulting overall productivity, through aspartate transcarbamylase activity. The highest influence on the pelagic environment was the strong difference in the...
Tipo: Text Palavras-chave: Rivers discharge; Phytoplankton distribution; Anchovy; Nutritional state; RNA/DNA ratio; Mesozooplankton; Metabolic descriptors; North-East Atlantic; Bay of Biscay.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00012/12290/9089.pdf
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Etude chémotaxonomique de la campagne TIC-MOC (mars 2015) – Projet CALHIS ArchiMer
Lampert, Luis; Perrot, Laurie; Delmas, Daniel.
A partir des échantillons collectés lors de la campagne TIC-MOC (mars 2015) sur le plateau continental argentin, des analyses en pigments phytoplanctoniques permettent de quantifier la distribution des principales classes algales par la méthode CHEMTAX. Les Prochlorophycées et cyanobactéries sont essentiellement dominantes dans les eaux chaudes du courant du Brésil, tandis que dans les eaux plus froides du courant des Malouines et la zone de mélange (T°< 20°C), c’est les Haptophytes (Phaeocystis et Emiliania huxleyi) qui dominent la biomasse chlorophyllienne. Des dominances moyennes en dinoflagellés supérieures 30% sont observées dans les deux masses d’eau.
Tipo: Text Palavras-chave: Chémotaxonomie; Coccolithophoridés; HPLC; Pigments phytoplanctoniques; Patagonie; Plateau continental.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00327/43784/43342.pdf
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Environmental conditions which lead to increase in cell density of the toxic dinoflagellates Dinophysis spp. in nutrient-rich and nutrient-poor waters of the French Atlantic coast ArchiMer
Delmas, Daniel; Herbland, Alain; Maestrini, Serge.
From 22 April to 19 June 1990, 7 stations located off La Rochelle, France, from near-shore ('bassin Marennes-Oleron') to offshore waters (50 m depth contour) were visited 8 times, with the aim of determining whether the toxic dinoflagellates Dinophysis spp. increase in cell density in nutrient-poor offshore water, in nutrient-rich inshore waters, or both, and to determine their vertical distribution in relation to stratification. Temperature stratification developed offshore, but not inshore. In April, few Dinophysis spp. were present offshore and none inshore. In late May, up to 15 X 10(3) cells l(-1) were recorded in the 10 to 15 m layer offshore, whereas inshore waters contained only a few cells l(-1). In late May, a short period of wind partly modified...
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/00190/30141/28772.pdf
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The role of phosphorus on planktonic production of the Gironde plume waters in the Bay of Biscay ArchiMer
Labry, Claire; Herbland, Alain; Delmas, Daniel.
More and more studies emphasize the status of phosphorus (P) as the principal limiting nutrient of phytoplankton growth, especially in coastal waters under the influence of freshwater discharges. The purpose of the present paper is to investigate the role of P on planktonic production in the waters influenced by the Gironde discharges; the Gironde being one of the two largest rivers on the French Atlantic coast. The survey is based on several cruises made in 1998 and 1999. Two different patterns were observed for waters with salinity below and above 34.5. For waters with salinity <34.5. P was found to be the first limiting nutrient of winter and spring phytoplankton blooms, based on undetectable phosphate (<20 nM), high NO3 : PO4 ratios, typically...
Tipo: Text Palavras-chave: Bay of Biscay; River plume; Salinity; Water quality analysis; Phytoplankton; Planktonic production.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-690.pdf
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Seasonal variation of riverine nutrient inputs in the northern Bay of Biscay (France), and patterns of marine phytoplankton response ArchiMer
Guillaud, Jean-francois; Aminot, Alain; Delmas, Daniel; Gohin, Francis; Lunven, Michel; Labry, Claire; Herbland, Alain.
Seasonal variations in nutrient inputs are described for the main rivers (Loire and Vilaine) flowing into the northern Bay of Biscay. The river plumes are high in N/P ratio in late winter and spring, but not in the inner plume during the summer. Conservative behavior results in most nutrients entering the estuary and eventually reaching the coastal zone. Temporal and spatial aspects of phytoplankton growth and nutrient uptake in the northern Bay of Biscay distinguish the central area of salinity 34 from the plume area. The first diatom bloom appears offshore in late winter, at the edge of the river plumes, taking advantage of haline stratification and anticyclonic "weather windows." In spring, when the central area of the northern shelf is...
Tipo: Text Palavras-chave: Northern Bay of Biscay; Phytoplankton; Continental shelf; Riverine inputs; Nutrients.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2007/publication-2354.pdf
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