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Fluorometric quantification of protoporphyrin IX in biological skin samples from in vitro penetration/permeation studies BJPS
Rossetti,Fábia Cristina; Depieri,Lívia Vieira; Tedesco,Antônio Cláudio; Bentley,Maria Vitória Lopes Badra.
A fluorometric analytical method was developed for quantification of protoporphyrin IX (PpIX) in skin samples and receptor phase solution after in vitro cutaneous penetration/permeation studies. Analytical conditions used were: excitation and emission wavelengths: 400 nm and 632 nm; bandwidth: 0.5 nm; excitation and emission slits: 10/10. PpIX was recovered from two different layers of skin, the stratum corneum (SC) and the epidermis plus dermis ([E+D]), by vortex homogenization, probe and bath sonication, using DMSO as an extraction solvent. The detection and quantification limits were 0.002 and 0.005 μg/mL, respectively. The assay was linear from 0.005 - 0.5 μg/mL. The within-day and between-day assay precision and accuracy in DMSO and receptor phase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fluorometric quantification; Protoporphyrin IX/determination; Photodynamic therapy; Porcine skin; Cutaneous penetration/in vitro study; Cutaneous permeation/in vitro study.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400017
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