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Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content R. Bras. Zootec.
Carvalho,Bruno Pena; Costa,Fernanda de Queirós; Detoni,Danielly; Rosa,Felipe Borges; Dias,Angelo José Burla.
ABSTRACT This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryotolerance; Polyunsaturated fatty acid; Vitrification.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100602
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Structural, hystochemical and cytochemical characteristics of the stigma and style in Passiflora edulis f. flavicarpa (Passifloraceae) BABT
Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Dias,Angelo José Burla; Ribeiro,Beatriz Ferreira; Viana,Alexandre Pio.
Studies were carried out on anatomic aspects of the stigma and style of yellow passionfruit. The stigmatic style consisted of papillae, many layers of sub-epidermal cells and a central transmitting tissue. The stigma was of dry, unbranched, multicellular multiseriate papillate type and the cells were highly vacuolated with walls that were relatively thin consisting of compactly arranged fibrils. The style was solid with a central core of transmitting tissue that had intercellular spaces containing a matrix that included polysaccharidic and lipidic substances. During development, cells of the transmitting tissue were arranged compactly while the mature pistil showed cells with a little more loosely arranged intercellular spaces. Both the mature stigma and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yellow passionfruit; Anatomy; Stigma and style; Histochemistry; Cytochemistry.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100011
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