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	Provedor de dados BJMBR (3) Autor Diniz,C.R. (3) De Lima,M.E. (2) Cordeiro,M.N. (1) Fortes-Dias,C.L. (1) Kattah,L.S. (1) Montes de Oca,H. (1) Santoro,M.M. (1) Santos,R.G. (1) Silva,L.M. (1) Mais... Palavra-chave Guinea pig ileum (2) Binding (1) Calcium channels (1) Cell culture (1) Cell line (1) Choline uptake (1) Crotalus durissus terrificus (1) Crotoxin (1) DAMD (1) DNA fingerprinting (1) Enteric nervous system (1) Phoneutria nigriventer (1) Phospholipase A2 (1) Spider toxins (1) Mais... Tipo do documento Info:eu-repo/semantics/other (2) Info:eu-repo/semantics/article (1) Ano 2001 (1) 2000 (1) 1999 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Crotoxin, the major toxin from the rattlesnake Crotalus durissus terrificus, inhibits ³H-choline uptake in guinea pig ileum BJMBR Kattah,L.S.; Santoro,M.M.; Diniz,C.R.; De Lima,M.E.. We examined the effect of crotoxin, the neurotoxic complex from the venom of the South American rattlesnake Crotalus durissus terrificus, on the uptake of ³H-choline in minces of smooth muscle myenteric plexus from guinea pig ileum. In the concentration range used (0.03-1 µM) and up to 10 min of treatment, crotoxin decreased ³H-choline uptake by 50-75% compared to control. This inhibition was time dependent and did not seem to be associated with the disruption of the neuronal membrane, because at least for the first 20 min of tissue exposure to the toxin (up to 1 µM) the levels of lactate dehydrogenase (LDH) released into the supernatant were similar to those of controls. Higher concentrations of crotoxin or more extensive incubation times with this toxin... Tipo: Info:eu-repo/semantics/other Palavras-chave: Crotoxin; Choline uptake; Guinea pig ileum; Crotalus durissus terrificus; Phospholipase A2. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900017 Binding sites and actions of Tx1, a neurotoxin from the venom of the spider Phoneutria nigriventer, in guinea pig ileum BJMBR Santos,R.G.; Diniz,C.R.; Cordeiro,M.N.; De Lima,M.E.. Tx1, a neurotoxin isolated from the venom of the South American spider Phoneutria nigriventer, produces tail elevation, behavioral excitation and spastic paralysis of the hind limbs after intracerebroventricular injection in mice. Since Tx1 contracts isolated guinea pig ileum, we have investigated the effect of this toxin on acetylcholine release, as well as its binding to myenteric plexus-longitudinal muscle membranes from the guinea pig ileum. [125I]-Tx1 binds specifically and with high affinity (Kd = 0.36 ± 0.02 nM) to a single, non-interacting (nH = 1.1), low capacity (Bmax 1.1 pmol/mg protein) binding site. In competition experiments using several compounds (including ion channel ligands), only PhTx2 and PhTx3 competed with [125I]-Tx1 for specific... Tipo: Info:eu-repo/semantics/other Palavras-chave: Phoneutria nigriventer; Spider toxins; Binding; Enteric nervous system; Calcium channels; Guinea pig ileum. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200019 Fingerprinting of cell lines by directed amplification of minisatellite-region DNA (DAMD) BJMBR Silva,L.M.; Montes de Oca,H.; Diniz,C.R.; Fortes-Dias,C.L.. The development of in vitro propagation of cells has been an extraordinary technical advance for several biological studies. The correct identification of the cell line used, however, is crucial, as a mistaken identity or the presence of another contaminating cell may lead to invalid and/or erroneous conclusions. We report here the application of a DNA fingerprinting procedure (directed amplification of minisatellite-region DNA), developed by Heath et al. [Nucleic Acids Research (1993) 21: 5782-5785], to the characterization of cell lines. Genomic DNA of cells in culture was extracted and amplified by PCR in the presence of VNTR core sequences, and the amplicons were separated by agarose gel electrophoresis. After image capture with a digital camera, the... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA fingerprinting; DAMD; Cell line; Cell culture. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100005 Registros recuperados: 3 Primeira ... 1 ... Última

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