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	Provedor de dados ArchiMer (3) Autor Diter, Alain (3) Dufy, Christian (2) Palavra-chave Bivalve (2) Cytochalasine B (2) Ruditapes philippinarum (2) Androgenesis (1) Androgenèse (1) Bivalves (1) Chlamys varis (1) Crassostrea gigas (1) Croissance larvaire (1) Cytochalasin B (1) Cytochalasin B. (1) Genetic (1) Génétique (1) Larval growth (1) Larval survival (1) Methods of gynogenesis (1) Méthode induction gynogenèse (1) Oncorhynchus mykiss (1) Polyploidy (1) Polyploïdie (1) Mais... Tipo do documento Text (3) Ano 1990 (3) País France (3) Idioma Inglês (2) Francês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Polyploidy in the Manila clam Ruditapes philippinarum. II. Chemical induction of tetraploid embryos ArchiMer Diter, Alain; Dufy, Christian. Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B. (CB). Two treatment times were found to be effective in inducing tetraplidy : 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced 64.4 ± 7.8% and 28.3 ± 2.3% tetraploid embrios, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment. Tipo: Text Palavras-chave: Ruditapes philippinarum; Bivalve; Tetraploidy; Cytochalasin B.; Ruditapes philippinarum; Bivalve; Tétraploïdie; Cytochalasine B. Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-2753.pdf Reproduction uniparentale et polyploidie induites chez la truite arc-en-ciel (Oncorhynchus mykiss) et chez les bivalves Crassostrea gigas, Ruditapes philippinarum et Chlamys varia ArchiMer Diter, Alain. Methods of gynogenesis, androgenesis and polyploidy induction were developed with the aim of genetic improvement in the rainbow trout, the Pacific oyster, the Manila clam and the black scallop. In the trout: · haploid androgenesis was induced by means of a pressure shock applied on diploid eggs, probably at the time of karyogamy. Diploid androgenetic individuals were directly produced from eggs fertilized with sperm from tetraploid male. · a heat shock treatment effective for suppressing the first egg mitosis was developed. This treatment allows the production of homozygous gynogenetic lines (checked using enzyme electrophoresis technique) from eggs inseminated with ultraviolet irradiated sperm. The treatment also induced tetraploidy in eggs normally... Tipo: Text Palavras-chave: Méthode induction gynogenèse; Androgenèse; Polyploïdie; Oncorhynchus mykiss; Crassostrea gigas; Ruditapes philippinarum; Chlamys varis; Génétique; Methods of gynogenesis; Androgenesis; Polyploidy; Oncorhynchus mykiss; Crassostrea gigas; Ruditapes philippinarum; Chlamys varis; Genetic. Ano: 1990 URL: http://archimer.ifremer.fr/doc/00210/32097/30553.pdf Polyploidy in the Manila clam Ruditapes philippinarum - 1 - Chemical induction and larval performances of triploids ArchiMer Dufy, Christian; Diter, Alain. An effective treatment using catochalasin B (1 mg CB/ml dimethylsulfoxyde in 1 litre of sea water) was developed to induce triploidy in the Manila clam Ruditapes philippinurum (Adams and Reeve). The percentage of induced triploidy was assessed by karyological examination of embryos and spat. The treatment was found to be optimal between 20 to 35 minutes post-insemination exposure to CB, at 25'C, and produced 75.8 ± 5.7% triploid embryos on the average over four experiments. Growth and survival of larvae to metamorphosis were recorded for two different rearings each split into two replicates. Both larval rearings showed similar survival up to settlement: 19.3 and 27.0%, on average over two replicates, in the treated batches relative to the controls. Mean... Tipo: Text Palavras-chave: Larval survival; Larval growth; Bivalves; Cytochalasin B; Triploidy; Sruvie larvaire; Croissance larvaire; Bivalve; Cytochalasine B; Triploïdie. Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-2752.pdf Registros recuperados: 3 Primeira ... 1 ... Última

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