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| Sprícigo,J.F.W.; Leme,L.O.; Guimarães,A.L.; Oliveira Neto,J.C.; Silva,P.C.P.; Moreira,N.H.; Pivato,I.; Silva,B.D.M.; Ramos,A.F.; Dode,M.A.N.. |
| ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming, blastocysts were in vitro cultured to assess re-expansion and hatching at 24 and 48 hours. Finally, at 48 hours, hatched blastocysts were submitted to RT-qPCR searching for BCL2A1, BAK, BAX and CASP3 genes. For MALDI-TOF, the ion intensity was expressed in arbitrary units. Blastocyst development was compared by Qui-square (P< 0.05). Among the most representative PL was the phosphatidylcholine [PC (32:0) + H]+; [PC (34:1) + H]+ and [PC (36:4) + H]+. Beyond the PL, MALDI revealed some triglycerides (TG), including PPL (50:2) + Na+, PPO... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig; Embryo; Cryopreservation; Lipids; Apoptosis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300837 |
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