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| Reis,Luciana Pimenta; Menezes,Liliane Denize Miranda; Lima,Graciela Kunrath; Santos,Ethiene Luiza de Souza; Dorneles,Elaine Maria Seles; Assis,Débora Cristina Sampaio de; Lage,Andrey Pereira; Cançado,Silvana de Vasconcelos; Figueiredo,Tadeu Chaves de. |
| ABSTRACT: In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS®30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS®30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C. jejuni; C. coli; Detection methods; VIDAS®30.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200451 |
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| Dorneles,Elaine Maria Seles; Pellegrin,Aiesca Oliveira; Péres,Igor Alexandre Hany Fuzeta Schabib; Mathias,Luis Antônio; Mourão,Guilherme; Bianchi,Rita de Cassia; Olifiers,Natalie; Rocha,Fabiana Lopes; Lage,Andrey Pereira. |
| A serological survey in free-ranging crab-eating foxes (Canidae: Cerdocyon thous) and brown-nosed coatis (Procyonidae: Nasua nasua) was performed in the Nhecolândia sub-region of the Brazilian Pantanal to evaluate the presence of anti-smooth Brucella antibodies on those wild populations. The detection of anti-smooth Brucella antibodies was performed by the Rose Bengal Test (RBT) as screening test and the Fluorescence Polarization Assay (FPA) as a confirmatory test. The frequency of smooth Brucella seropositive animals were 13.2% (5/38, 95% CI: 4.4% - 28.1%) for crab-eating foxes and 8.8% (3/34, 95% CI: 1.9% -23.7%) for brown-nosed coatis. No association was found between seropositivity for brucellosis and gender or age. The results of this study suggest... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Brazil; Brucella; Pantanal; Cerdocyon thous; Nasua nasua; Reservoirs; Epidemiology. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001202193 |
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