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	Provedor de dados Genet. Mol. Biol. (4) Braz. J. Genet. (1) Autor Drets,Máximo E. (5) Santiñaque,Federico F. (2) Bonomi,Rossana (1) Folle,Gustavo A. (1) Martínez-López,Wilner (1) Mendizábal,Marcos (1) Obe,Günter (1) Mais... Palavra-chave AluI (1) BamHI (1) Chinese hamster (1) Chromatid gaps (1) Chromatin structure (1) Chromosome nomenclature (1) Human chromosomes (1) Microdensitometry (1) Microphotometric scanning (1) Minute telomeric SCEs (1) Ovary cells (1) T-banding (1) Telomere (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Ano 2007 (1) 2006 (1) 2000 (1) 1998 (1) 1996 (1) País Brazil (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Non-random distribution of high density chromatin detected at opposite ends of T-banded human metaphase chromosomes Genet. Mol. Biol. Santiñaque,Federico F.; Drets,Máximo E.. Previous research using microdensitometric scanning and computer graphic image analysis showed that T-banded segments of human metaphase chromosomes usually exhibit an asymmetrical distribution of high density (HD) chromatin between sister chromatids. Here, we employed the same methods to analyze HD chromatin distribution at opposite ends of T-banded human lymphocyte chromosomes. This study revealed that in most chromosomes with an asymmetrical distribution of HD chromatin at both ends, the highest densities of each arm were located in opposite chromatids. The frequency of this configuration was 0.792 per chromosome, indicating that the highest chromatin densities of the terminal segments of T-banded human chromosomes were non-randomly distributed at... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human chromosomes; T-banding; Chromatin structure; Telomere; Microdensitometry. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600004 Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells Genet. Mol. Biol. Drets,Máximo E.; Mendizábal,Marcos. Microphotometrical scanning and computer graphic image analysis were carried out to detect the distribution of chromatin densities in subtelomeric segments of T-banded endoreduplicated chromosomes of Chinese hamster ovary (CHO) cells. Chromatin density patterns detected with this method were similar to those previously found in CHO and normal human chromosomes. The highest chromatin densities were considered as marker segments which led to the detection of reciprocal changes of position in endoreduplicated chromosomes during cell spreading on the slide. The problem of the subtelomeric T-banding density patterns found in the endoreduplicated chromosomes and their relation to the structure and molecular composition of this region is briefly discussed. Tipo: Info:eu-repo/semantics/article Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200008 Insights into the structure of the subtelomeric chromosome segments Genet. Mol. Biol. Drets,Máximo E.. Tipo: Info:eu-repo/semantics/article Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400056 A proposal of a standardised nomenclature for terminal minute sister chromatid exchanges Genet. Mol. Biol. Drets,Máximo E.; Santiñaque,Federico F.; Obe,Günter. We described spontaneous minute sister chromatid exchanges (SCE) in telomeric regions of human and Chinese hamster ovary (CHO) chromosomes more than 10 years ago. These structures, which we called t-SCE, were detected by means of highly precise quantitative microphotometrical scanning and computer graphic image analysis. Recently, several authors using the CO-FISH method also found small SCEs in telomeric regions and called them T-SCE. The use of different terms for designating the same phenomenon should be avoided. We propose ter SCE as a uniform nomenclature for minute telomeric SCEs. Tipo: Info:eu-repo/semantics/article Palavras-chave: Minute telomeric SCEs; Chromosome nomenclature. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300007 Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells Braz. J. Genet. Martínez-López,Wilner; Bonomi,Rossana; Folle,Gustavo A.; Drets,Máximo E.. Chromatid gaps and breaks induced by the restriction endonucleases AluI and BamHI in the long arm of chromosome 1 of Chinese hamster ovary cells were microphotometrically scanned and mapped to a quantitative G-band map. More than 50% of chromatid breaks appeared as chromatin losses of greater than 5% of the total arm length. The majority of chromatid gaps and breaks as well as chromatin losses induced by both restriction endonucleases were non-randomly located in a region from 0.35 to 0.65 relative length units of the long arm of chromosome 1. We suggest that the access of these endonucleases to chromosomal DNA depends on the local organization of the chromatin. Tipo: Info:eu-repo/semantics/article Palavras-chave: Microphotometric scanning; Chromatid gaps; AluI; BamHI; Chinese hamster; Ovary cells. Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400007 Registros recuperados: 5 Primeira ... 1 ... Última

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