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| Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain. |
| Marine organisms represent a rather unexplored source of new activity and biological functions of molecules for biotechnologies. The research in chemical ecology of the marine environment leads to looking at metabolites of recognition or defence produced by these models. Marine bacteria belonging to the Pseudoalteromonas genus of the Gammaproteobacteria class are often found in association with marine eukaryotes, and their ability to produce a variety of biological activities attracted a particular attention. The marine Pseudoalteromonas sp. 3J6 and D41 were selected for their capacity to inhibit the biofilm formation of other bacteria. The study of antibiofilm metabolites synthesised by marine bacteria 3J6 and D41 biofilms can lead to the development of... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00089/19990/17666.pdf |
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| Jouault, Albane; Gobet, Angelique; Simon, Marjolaine; Portier, Emilie; Perennou, Morgan; Corre, Erwan; Gaillard, Fanny; Vallenet, David; Michel, Gurvan; Fleury, Yannick; Bazire, Alexis; Dufour, Alain. |
| The aim was to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another non-active Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein including a predicted 20-residue... |
| Tipo: Text |
Palavras-chave: Antibiofilm protein; Pseudoalteromonas; Pseudomonas aeruginosa; Biofilm. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75517/76405.pdf |
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| Boyen, Catherine; Jaouen, Pascal; Blanchard, Gilbert; Compere, Chantal; Dufour, Alain; Durand, Patrick; Guerard, Fabienne; Hallouin, Florence; Jebbar, Mohamed; Le Blay, Gwenaelle; Le Deit, Hervé; Le Seyec, Jocelyne; Monks, Brian; Portal-sellin, Rachel; Probert, Ian; Pruvost, Jeremy. |
| Marine (= blue) biotechnology, i.e. the utilization of marine bio-resources as a target or source of biotechnological applications, is a field with massive potential for innovation and economic growth. In a context of rapid climate change and increasing pressure on natural resources, renewed interest in marine biotechnology has been promoted by application of recent methodological and technological advances, notably in bioprocessing and in the various –omics domains, to the study of marine biodiversity. Marine biological resources potentially represent a sustainable raw material for exploitation in diverse fields, including nutrition, health, agriculture, aquaculture, energy, environment, and cosmetics. Marine biotechnology is now recognized as a... |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00273/38460/36860.pdf |
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| Rodrigues, Sophie; Paillard, Christine; Van Dillen, Sabine; Tahrioui, Ali; Berjeaud, Jean-marc; Dufour, Alain; Bazire, Alexis. |
| Marine pathogenic bacteria are able to form biofilms on many surfaces, such as mollusc shells, and they can wait for the appropriate opportunity to induce their virulence. Vibrio tapetis can develop such biofilms on the inner surface of shells of the Ruditapes philippinarum clam, leading to the formation of a brown conchiolin deposit in the form of a ring, hence the name of the disease: Brown Ring Disease. The virulence of V. tapetis is presumed to be related to its capacity to form biofilms, but the link has never been clearly established at the physiological or genetic level. In the present study, we used RNA-seq analysis to identify biofilm- and virulence-related genes displaying altered expression in biofilms compared to the planktonic condition. A... |
| Tipo: Text |
Palavras-chave: Biofilm; Virulence; Vibrio tapetis; Transcriptome; Quorum sensing; Type VI secretion system; Brown ring disease. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00470/58140/60611.pdf |
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| Ritter, Andres; Com, Emmanuelle; Bazire, Alexis; Dos Santos Goncalves, Marina; Pineau, Charles; Compere, Chantal; Dufour, Alain; Potin, Philippe. |
| Biofouling is ubiquitous in marine environment, and bacteria are among the first organisms to foul surfaces. They form biofilms which serve as focus for the attachment and growth of other organisms, such as invertebrates, sessile plants, and animals (Davis et al., 1989). Mature marine biofouling communities are complex, highly dynamic ecosystems (Fig.1) and once established are extremely difficult to eradicate (Holmstrom et al., 2002). For this reason the understanding of the mechanisms leading to marine bacterial attachment and its subsequent biofilm development are of great biological importance with obvious potential industrial outcomes. This development is conditioned by complex processes involving bacterial attachment to surfaces, growth, cell-to-cell... |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00089/19987/17651.pdf |
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| Klein, Geraldine; Dufour, Alain; Compere, Chantal. |
| Lors d’une immersion en eau de mer, tous les matériaux et structures sont rapidement colonisés par des salissures d’origine biologique : des biofilms. La transformation des propriétés de surface dumatériau et la présence demicro- oumacro-organismes (bactéries, algues, balanes, larves) engendrent des risques accrus de corrosion localisée, la biodétérioration desmatériaux immergés, le blocage des fonctions mécaniques…Afin de contrôler le dépôt et le développement de ces biofilms, la méthode privilégiée est l’application de peintures anti-salissures. Cependant ces revêtements sont généralement toxiques pour l’environnement. C’est pourquoi, les travaux menés actuellement visent à isoler de nouvelles molécules, produites par les bactéries marines, dans le but... |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00351/46213/45910.pdf |
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| Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain. |
| Bacterial biofilms, frequently in association with algae, protozoa and fungi, are found on all submerged structures in the marine environment. Biofilms are responsible for a range of surface‐associated and diffusible signals, which may moderate the settling behaviour of cells, spores and larvae. Thus, marine microorganisms are a new source of bioactive compounds, which enhance or inhibit the settlement of organisms. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00089/20005/17667.pdf |
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