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| Hogrel, Gaelle; Lu, Yang; Alexandre, Nicolas; Bossé, Audrey; Dulermo, Remi; Ishino, Sonoko; Ishino, Yoshizumi; Flament, Didier. |
| Among the three domains of life, the process of homologous recombination (HR) plays a central role in the repair of double-strand DNA breaks and the restart of stalled replication forks. Curiously, main protein actors involved in the HR process appear to be essential for hyperthermophilic Archaea raising interesting questions about the role of HR in replication and repair strategies of those Archaea living in extreme conditions. One key actor of this process is the recombinase RadA, which allows the homologous strand search and provides a DNA substrate required for following DNA synthesis and restoring genetic information. DNA polymerase operation after the strand exchange step is unclear in Archaea. Working with Pyrococcus abyssi proteins, here we show... |
| Tipo: Text |
Palavras-chave: Homologous recombination; Archaea; DNA polymerase; Recombinase; DNA repair. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00641/75274/75539.pdf |
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| Dulermo, Remi; Brunel, Francois; Dulermo, Thierry; Ledesma-amaro, Rodrigo; Vion, Jeremy; Trassaert, Marion; Thomas, Stephane; Nicaud, Jean-marc; Leplat, Christophe. |
| Background The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and the hybrid hp4d promoter. Recently, new hp4d-inspired promoters have been created that couple various numbers of UAS1 tandem elements with the minimal LEU2 promoter or the TEF promoter. Three different protein-secretion signaling sequences can be used: preLip2, preXpr2, and preSuc2. Results To our knowledge, our study is the first to use a set of vectors with promoters of variable strength to produce proteins of industrial interest. We used the more conventional TEF and hp4d promoters along with five new hybrid promoters: 2UAS1-pTEF,... |
| Tipo: Text |
Palavras-chave: Yarrowia lipolytica; Protein production; RedStar2; Glucoamylase; Xylanase; Hybrid promoters. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00428/54003/55193.pdf |
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| Hogrel, Gaelle; Lu, Yang; Laurent, Sebastien; Henry, Etienne; Etienne, Clarisse; Duy Khanh Phung,; Dulermo, Remi; Bosse, Audrey; Pluchon, Pierre Francois; Clouet-d'Orval, Beatrice; Flament, Didier. |
| Several archaeal species prevalent in extreme environments are particularly exposed to factors likely to cause DNA damages. These include hyperthermophilic archaea (HA), living at temperatures >70°C, which arguably have efficient strategies and robust genome guardians to repair DNA damage threatening their genome integrity. In contrast to Eukarya and other archaea, homologous recombination appears to be a vital pathway in HA, and the Mre11–Rad50 complex exerts a broad influence on the initiation of this DNA damage response process. In a previous study, we identified a physical association between the Proliferating Cell Nuclear Antigen (PCNA) and the Mre11–Rad50 (MR) complex. Here, by performing co-immunoprecipitation and SPR analyses, we identified a... |
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| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00439/55092/56534.pdf |
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