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Incidence and clinical characteristics of the infection by the Respiratory Syncytial Virus in children admitted in Santa Casa de São Paulo Hospital BJID
Pecchini,Rogério; Berezin,Eitan N.; Felício,Maria C. Calahani; Passos,Saulo D.; Souza,Maria Cândido O. de; Lima,Lourdes Rehder de Andrade Vaz de; Ueda,Mirthes; Matsumoto,Tokiko Kyomen; Durigon,Edison L..
The purpose of this study was to identify the rate of infections due to RSV and other viruses in children. In addition we have analyzed demographic data and clinical characteristics of the RSV-positive patients comparing with patients infected by other respiratory viruses. We also described the seasonality of the RSV occurrence in a hospital in São Paulo. Children below 5 years old admitted in Santa Casa de São Paulo Hospital between February 2005 and September 2006 due to acute respiratory infections (ARI) were included. A nasopharyngeal specimens were obtained with sterile No. 5 French feeding catheters as soon as possible (usually within 24 h). Specimens were kept refrigerated at 4ºC and transported to Adolfo Lutz Institute, where the indirect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Children; Bronchiolitis; RSV; Respiratory infections.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600006
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Polymerase chain reaction detection of adenovirus DNA sequences in human lymphocytes BJM
Araújo,Aufra A.; Yokosawa,Jonny; Durigon,Edison L.; Ventura,Armando M..
Human lymphoid cells frequently carry adenoviruses and DNA sequences have been identified in peripheral blood lymphocytes by Southern blot and PCR, although these cells are not permissive for virus replication, suggesting persistence of the viral genome. In order to investigate this phenomenon we screened non-symptomatic volunteers for adenovirus DNA presence and E1A gene expression. DNA samples extracted from peripheral blood mononuclear cells of 51 volunteers were submitted to PCR using primers for a conserved hexon sequence, followed by nested PCR. Adenovirus sequences were detected in 27 samples (52.9%). After more than one year, new samples of these positive volunteers were analyzed and in 70.8% of the cases the result was maintained. Since this could...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenoviral infection; Viral persistence; Lymphocytes; Nested PCR assay.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200017
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