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Simultaneous production of amylases and proteases by Bacillus subtilis in brewery wastes BJM
Blanco,Alina Sánchez; Durive,Osmar Palacios; Pérez,Sulema Batista; Montes,Zoraida Díaz; Guerra,Nelson Pérez.
ABSTRACT The simultaneous production of amylase (AA) and protease (PA) activity by Bacillus subtilis UO-01 in brewery wastes was studied by combining the response surface methodology with the kinetic study of the process. The optimum conditions (T = 36.0 °C and pH = 6.8) for high biomass production (0.92 g/L) were similar to the conditions (T = 36.8 °C and pH = 6.6) for high AA synthesis (9.26 EU/mL). However, the maximum PA level (9.77 EU/mL) was obtained at pH 7.1 and 37.8 °C. Under these conditions, a considerably high reduction (between 69.9 and 77.8%) of the initial chemical oxygen demand of the waste was achieved. In verification experiments under the optimized conditions for production of each enzyme, the AA and PA obtained after 15 h of incubation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylases; Bacillus subtilis; Brewery wastes; Proteases; Response surface methodology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300665
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