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| Registros recuperados: 22 | |
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| Duval, David; Galinier, Richard; Mouahid, Gabriel; Toulza, Eve; Allienne, Jean Francois; Portela, Julien; Calvayrac, Christophe; Rognon, Anne; Arancibia, Nathalie; Mitta, Guillaume; Theron, Andre; Gourbal, Benjamin. |
| Background Schistosomiasis is the second-most widespread tropical parasitic disease after malaria. Various research strategies and treatment programs for achieving the objective of eradicating schistosomiasis within a decade have been recommended and supported by the World Health Organization. One of these approaches is based on the control of snail vectors in endemic areas. Previous field studies have shown that competitor or predator introduction can reduce snail numbers, but no systematic investigation has ever been conducted to identify snail microbial pathogens and evaluate their molluscicidal effects. Methodology/Principal findings In populations of Biomphalaria glabrata snails experiencing high mortalities, white nodules were visible on snail... |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00615/72716/71712.pdf |
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| Geyer, Kathrin K.; Niazi, Umar H.; Duval, David; Cosseau, Celine; Tomlinson, Chad; Chalmers, Iain W.; Swain, Martin T.; Cutress, David J.; Bickham-wright, Utibe; Munshi, Sabrina E.; Grunau, Christoph; Yoshino, Timothy P.; Hoffmann, Karl F.. |
| Background The debilitating human disease schistosomiasis is caused by infection with schistosome parasites that maintain a complex lifecycle alternating between definitive (human) and intermediate (snail) hosts. While much is known about how the definitive host responds to schistosome infection, there is comparably less information available describing the snail's response to infection. Methodology/Principle findings Here, using information recently revealed by sequencing of the Biomphalaria glabrata intermediate host genome, we provide evidence that the predicted core snail DNA methylation machinery components are associated with both intra-species reproduction processes and inter-species interactions. Firstly, methyl-CpG binding domain protein... |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00389/50000/71812.pdf |
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| Tetreau, Guillaume; Dhinaut, Julien; Galinier, Richard; Audant-lacour, Pascaline; Voisin, Sébastien N; Arafah, Karim; Chogne, Manon; Hilliou, Frédérique; Bordes, Anaïs; Sabarly, Camille; Chan, Philippe; Walet-balieu, Marie-laure; Vaudry, David; Duval, David; Bulet, Philippe; Coustau, Christine; Moret, Yannick; Gourbal, Benjamin. |
| In a number of species, individuals exposed to pathogens can mount an immune response and transmit this immunological experience to their offspring, thereby protecting them against persistent threats. Such vertical transfer of immunity, named trans-generational immune priming (TGIP), has been described in both vertebrates and invertebrates. Although increasingly studied during the last decade, the mechanisms underlying TGIP in invertebrates are still elusive, especially those protecting the earliest offspring life stage, i.e. the embryo developing in the egg. In the present study, we combined different proteomic and transcriptomic approaches to determine whether mothers transfer a “signal” (such as fragments of infecting bacteria), mRNA and/or... |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00655/76684/77815.pdf |
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| De Carvalho Augusto, Ronaldo; Rey, Oliver; Cosseau, Céline; Chaparro, Cristian; Vidal-dupiol, Jeremie; Allienne, Jean-françois; Duval, David; Pinaud, Silvain; Tönges, Sina; Andriantsoa, Ranja; Luquet, Emilien; Aubret, Fabien; Dia Sow, Mamadou; David, Patrice; Thomson, Vicki; Joly, Dominique; Gomes Lima, Mariana; Federico, Déborah; Danchin, Etienne; Minoda, Aki; Grunau, Christoph. |
| We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies, and the analysis of alignment results. We show that the protocol can be used to monitor chromatin structure changes in populations when exposed to environmental cues. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size (~10,000 cells), and it requires little molecular biology handling expertise. |
| Tipo: Text |
Palavras-chave: Epigenetics; Epigenomics; ATAC-seq; Daphnia pulex; Schistosoma mansoni. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00681/79353/81859.pdf |
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| Adema, Coen M.; Hillier, Ladeana W.; Jones, Catherine S.; Loker, Eric S.; Knight, Matty; Minx, Patrick; Oliveira, Guilherme; Raghavan, Nithya; Shedlock, Andrew; Do Amaral, Laurence Rodrigues; Arican-goktas, Halime D.; Assis, Juliana G.; Baba, Elio Hideo; Baron, Olga L.; Bayne, Christopher J.; Bickham-wright, Utibe; Biggar, Kyle K.; Blouin, Michael; Bonning, Bryony C.; Botka, Chris; Bridger, Joanna M.; Buckley, Katherine M.; Buddenborg, Sarah K.; Caldeira, Roberta Lima; Carleton, Julia; Carvalho, Omar S.; Castillo, Maria G.; Chalmers, Iain W.; Christensens, Mikkel; Clifton, Sandra; Cosseau, Celine; Coustau, Christine; Cripps, Richard M.; Cuesta-astroz, Yesid; Cummins, Scott F.; Di Stephano, Leon; Dinguirard, Nathalie; Duval, David; Emrich, Scott; Feschotte, Cedric; Feyereisen, Rene; Fitzgerald, Peter; Fronick, Catrina; Fulton, Lucinda; Galinier, Richard; Gava, Sandra G.; Geusz, Michael; Geyer, Kathrin K.; Giraldo-calderon, Gloria I.; Gomes, Matheus De Souza; Gordy, Michelle A.; Gourbal, Benjamin; Grunau, Christoph; Hanington, Patrick C.; Hoffmann, Karl F.; Hughes, Daniel; Humphries, Judith; Jackson, Daniel J.; Jannotti-passos, Liana K.; Jeremias, Wander De Jesus; Jobling, Susan; Kamel, Bishoy; Kapusta, Aurelie; Kaur, Satwant; Koene, Joris M.; Kohn, Andrea B.; Lawson, Dan; Lawton, Scott P.; Liang, Di; Limpanont, Yanin; Liu, Sijun; Lockyer, Anne E.; Lovato, Tyanna L.; Ludolf, Fernanda; Magrini, Vince; Mcmanus, Donald P.; Medina, Monica; Misra, Milind; Mitta, Guillaume; Mkoji, Gerald M.; Montague, Michael J.; Montelongo, Cesar; Moroz, Leonid L.; Munoz-torres, Monica C.; Niazi, Umar; Noble, Leslie R.; Oliveira, Francislon S.; Pais, Fabiano S.; Papenfuss, Anthony T.; Peace, Rob; Pena, Janeth J.; Pila, Emmanuel A.; Quelais, Titouan; Raney, Brian J.; Rast, Jonathan P.; Rollinson, David; Rosse, Izinara C.; Rotgans, Bronwyn; Routledge, Edwin J.; Ryan, Kathryn M.; Scholte, Larissa L. S.; Storey, Kenneth B.; Swain, Martin; Tennessen, Jacob A.; Tomlinson, Chad; Trujillo, Damian L.; Volpi, Emanuela V.; Walker, Anthony J.; Wang, Tianfang; Wannaporn, Ittiprasert; Warren, Wesley C.; Wu, Xiao-jun; Yoshino, Timothy P.; Yusuf, Mohammed; Zhang, Si-ming; Zhao, Min; Wilson, Richard K.. |
| Biomphalaria snails are instrumental in transmission of the human blood fluke Schistosoma mansoni. With the World Health Organization's goal to eliminate schistosomiasis as a global health problem by 2025, there is now renewed emphasis on snail control. Here, we characterize the genome of Biomphalaria glabrata, a lophotrochozoan protostome, and provide timely and important information on snail biology. We describe aspects of phero-perception, stress responses, immune function and regulation of gene expression that support the persistence of B. glabrata in the field and may define this species as a suitable snail host for S. mansoni. We identify several potential targets for developing novel control measures aimed at reducing snail-mediated transmission of... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00387/49840/71816.pdf |
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| Portet, Anais; Galinier, Richard; Pinaud, Silvain; Portela, Julien; Nowacki, Fanny; Gourbal, Benjamin; Duval, David. |
| Insect thioester-containing protein (iTEP) is the most recently defined group among the thioester-containing protein (TEP) superfamily. TEPs are key components of the immune system, and iTEPs from flies and mosquitoes were shown to be major immune weapons. Initially characterized from insects, TEP genes homologous to iTEP were further described from several other invertebrates including arthropods, cniderians, and mollusks albeit with few functional characterizations. In the freshwater snail Biomphalaria glabrata, a vector of the schistosomiasis disease, the presence of a TEP protein (BgTEP) was previously described in a well-defined immune complex involving snail lectins (fibrinogen-related proteins) and schistosome parasite mucins (SmPoMuc). To... |
| Tipo: Text |
Palavras-chave: Thioester-containing protein; Complement-like protein; Biomphalaria glabrata; Interaction host/pathogens; Innate immunity. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00444/55548/71780.tif |
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| Portet, Anais; Pinaud, Silvain; Chaparro, Cristian; Galinier, Richard; Dheilly, Nolwenn M.; Portela, Julien; Charriere, Guillaume; Allienne, Jean-francois; Duval, David; Gourbal, Benjamin. |
| Selective pressures between hosts and their parasites can result in reciprocal evolution or adaptation of specific life history traits. Local adaptation of resident hosts and parasites should lead to increase parasite infectivity/virulence (higher compatibility) when infecting hosts from the same location (in sympatry) than from a foreign location (in allopatry). Analysis of geographic variations in compatibility phenotypes is the most common proxy used to infer local adaptation. However, in some cases, allopatric host-parasite systems demonstrate similar or greater compatibility than in sympatry. In such cases, the potential for local adaptation remains unclear. Here, we study the interaction between Schistosoma and its vector snail Biomphalaria in which... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00488/59982/63214.pdf |
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| Alba, Annia; Vazquez, Antonio A.; Sanchez, Jorge; Duval, David; Hernandez, Hilda M.; Sabourin, Emeline; Vittecoq, Marion; Hurtrez-bousses, Sylvie; Gourbal, Benjamin. |
| Background: Pseudosuccinea columella is one of the most widespread vectors of Fasciola hepatica, a globally distributed trematode that affects humans, livestock and wildlife. The exclusive occurrence in Cuba of susceptible and naturally-resistant populations to F. hepatica within this snail species, offers a fascinating model for evolutionary biology, health sciences and vector control strategies. In particular, resistance in P. columella is characterized by the encapsulation of the parasite by host's immune cells and has been experimentally tested using different Cuban F. hepatica isolates with no records of successful infection. Here, we aimed to explore for the first time, the effect of different parasite doses, successive exposures and different... |
| Tipo: Text |
Palavras-chave: Snail-trematode interaction; Lymnaeidae; Liver fluke; Experimental infection; Immune priming; Compatibility; Allopatric parasites. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00467/57894/71760.pdf |
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| Pinaud, Silvain; Portela, Julien; Duval, David; Nowacki, Fanny C.; Olive, Marie-aude; Allienne, Jean-francois; Galinier, Richard; Dheilly, Nolwenn M.; Kieffer-jaquinod, Sylvie; Mitta, Guillaume; Theron, Andre; Gourbal, Benjamin. |
| Discoveries made over the past ten years have provided evidence that invertebrate antiparasitic responses may be primed in a sustainable manner, leading to the failure of a secondary encounter with the same pathogen. This phenomenon called "immune priming" or "innate immune memory" was mainly phenomenological. The demonstration of this process remains to be obtained and the underlying mechanisms remain to be discovered and exhaustively tested with rigorous functional and molecular methods, to eliminate all alternative explanations. In order to achieve this ambitious aim, the present study focuses on the Lophotrochozoan snail, Biomphalaria glabrata, in which innate immune memory was recently reported. We provide herein the first evidence that a shift from a... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00315/42664/71872.pdf |
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| Vidal-dupiol, Jeremie; Ladriere, Ophelie; Destoumieux-garzon, Delphine; Sautiere, Pierre-eric; Meistertzheim, Anne-leila; Tambutte, Eric; Tambutte, Sylvie; Duval, David; Foure, Laurent; Adjeroud, Mehdi; Mitta, Guillaume. |
| Scleractinian corals are the most basal eumetazoan taxon and provide the biological and physical framework for coral reefs, which are among the most diverse of all ecosystems. Over the past three decades and coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent and severe diseases, which are now geographically widespread and a major threat to these ecosystems. Although coral immunity has been the subject of increasing study, the available information remains fragmentary, especially with respect to coral antimicrobial responses. In this study, we characterized damicornin from Pocillopora damicornis, the first scleractinian antimicrobial peptide (AMP) to be reported. We found that its precursor has... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00039/14996/12433.pdf |
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| Aliaga, Benoit; Bulla, Ingo; Mouahid, Gabriel; Duval, David; Grunau, Christoph. |
| provides just a set of environment dependent instructions, or whether it is integral part of an inheritance system. We argued that in the latter case the epigenetic code should share the universality quality of the genetic code. We focused on DNA methylation. Since availability of DNA methylation data is biased towards model organisms we developed a method that uses kernel density estimations of CpG observed/expected ratios to infer DNA methylation types in any genome. We show here that our method allows for robust prediction of mosaic and full gene body methylation with a PPV of 1 and 0.87, respectively. We used this prediction to complement experimental data, and applied hierarchical clustering to identify methylation types in similar to 150 eucaryotic... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00479/59040/61671.pdf |
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| Huot, Camille; Clerissi, Camille; Gourbal, Benjamin; Galinier, Richard; Duval, David; Toulza, Eve. |
| Planorbidae snails are the intermediate host for the trematode parasite of the Schistosoma genus, which is responsible for schistosomiasis, a disease that affects both humans and cattle. The microbiota for Schistosoma has already been described as having an effect on host/parasite interactions, specifically through immunological interactions. Here, we sought to characterize the microbiota composition of seven Planorbidae species and strains. Individual snail microbiota was determined using 16S ribosomal DNA amplicon sequencing. The bacterial composition was highly specific to the host strain with limited interindividual variation. In addition, it displayed complete congruence with host phylogeny, revealing a phylosymbiosis pattern. These results were... |
| Tipo: Text |
Palavras-chave: Microbiota; Phylosymbiosis; Metabarcoding; Planorbid snails; Tripartite interactions; Schistosomiasis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71807/70295.pdf |
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| Lassalle, Damien; Tetreau, Guillaume; Pinaud, Silvain; Galinier, Richard; Crickmore, Neil; Gourbal, Benjamain; Duval, David. |
| Biomphalaria glabrata is a freshwater Planorbidae snail. In its environment, this mollusk faces numerous microorganisms or pathogens, and has developed sophisticated innate immune mechanisms to survive. The mechanisms of recognition are quite well understood in Biomphalaria glabrata, but immune effectors have been seldom described. In this study, we analyzed a new family of potential immune effectors and characterized five new genes that were named Glabralysins. The five Glabralysin genes showed different genomic structures and the high degree of amino acid identity between the Glabralysins, and the presence of the conserved ETX/MTX2 domain, support the hypothesis that they are pore-forming toxins. In addition, tertiary structure prediction confirms that... |
| Tipo: Text |
Palavras-chave: Pore-forming toxin; Cry toxin; Invertebrate; Biomphalaria glabrata; Host; Pathogen; Vector snail; Innate immunity. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00601/71267/69655.pdf |
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| De Carvalho Augusto, Ronaldo; Duval, David; Grunau, Christoph. |
| Evidence of how environmental cues affect the phenotypes of, and compatibility between Schistosoma mansoni and their hosts come from studies in environmental parasitology and research on host diet and chemotherapeutic treatment. Schistosomes deal with a multitude of signals from the water environment as well as cues that come from their hosts, particularly in response to molecules that serve to recognize and destroy them, i.e., those molecules that arise from their hosts' immune systems. These interactions shape, not only the parasite's morphology, metabolism and behavior in the short-term, but also their infection success and development into different stage-specific phenotypes later in their life cycle, through the modification of the parasite's... |
| Tipo: Text |
Palavras-chave: Schistosomiasis; Environmental cues; Inheritance systems; Imune response; Host-para site interactions. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00508/61941/66028.pdf |
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| Duval, David; Pichon, Rémi; Lassalle, Damien; Laffitte, Maud; Gourbal, Benjamain; Galinier, Richard. |
| Thioester-containing proteins (TEPs) superfamily is known to play important innate immune functions in a wide range of animal phyla. TEPs are involved in recognition, and in the direct or mediated killing of several invading organisms or pathogens. While several TEPs have been identified in many invertebrates, only one TEP (named BgTEP) has been previously characterized in the freshwater snail, Biomphalaria glabrata. As the presence of a single member of that family is particularly intriguing, transcriptomic data and the recently published genome were used to explore the presence of other BgTEP related genes in B. glabrata. Ten other TEP members have been reported and classified into different subfamilies: Three complement-like factors (BgC3-1 to BgC3-3),... |
| Tipo: Text |
Palavras-chave: Biomphalaria glabrata; Thioester-containing protein; Alternative splicing; Immunity; Schistosome. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00601/71268/69657.pdf |
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| De Carvalho Augusto, Ronaldo; Merad, Nadjiya; Rognon, Anne; Gourbal, Benjamin; Bertrand, Cédric; Djabou, Nassim; Duval, David. |
| Background Freshwater snails are the intermediate hosts of a large variety of trematode flukes such as Schistosoma mansoni responsible for one of the most important parasitic diseases caused by helminths, affecting 67 million people worldwide. Recently, the WHO Global Vector Control Response 2017–2030 (GVCR) programme reinforced its message for safer molluscicides as part of required strategies to strengthen vector control worldwide. Here, we present the essential oil from Eryngium triquetrum as a powerful product with molluscicide and parasiticide effect against S. mansoni and the snail intermediate host Biomphalaria glabrata. Methods In the present study, we describe using several experimental approaches, the chemical composition of E. triquetrum... |
| Tipo: Text |
Palavras-chave: Biomphalaria glabrata; Eryngium triquetrum; Molluscicide; Oil; Parasiticide; Schistosoma mansoni. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00652/76382/77391.pdf |
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| Fneich, Sara; Theron, Andre; Cosseau, Celine; Rognon, Anne; Aliaga, Benoit; Buard, Jerome; Duval, David; Arancibia, Nathalie; Boissier, Jerome; Roquis, David; Mitta, Guillaume; Grunau, Christoph. |
| Background: Adaptive evolution is not possible without the generation of phenotypic variants. The origin of these variations has been a central topic in evolutionary biology. Up to now, it was commonly accepted that standing genetic variation is the only cause of phenotypic variants. However, epigenetic information is emerging as a complementary source of heritable phenotypic variation that contributes to evolution. The relative importance of genetics and epigenetics in generating heritable phenotypic variation is nevertheless a matter of debate. Results: We used a host-parasite system to address this question. The human blood fluke Schistosoma mansoni can adapt rapidly to new intermediate snail hosts. The interaction between parasite and mollusk is... |
| Tipo: Text |
Palavras-chave: Epigenetics; Adaptive evolution; Compatibility polymorphism; Schistosoma mansoni. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00615/72708/71730.pdf |
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| Pinaud, Silvain; Tetreau, Guillaume; Poteaux, Pierre; Galinier, Richard; Chaparro, Cristian; Lassalle, Damien; Portet, Anaïs; Simphor, Elodie; Gourbal, Benjamin; Duval, David. |
| Aerolysins initially characterized as virulence factors in bacteria are increasingly found in massive genome and transcriptome sequencing data from metazoans. Horizontal gene transfer has been demonstrated as the main way of aerolysin-related toxins acquisition in metazoans. However, only few studies have focused on their potential biological functions in such organisms. Herein, we present an extensive characterization of a multigene family encoding aerolysins - named biomphalysin - in Biomphalaria glabrata snail, the intermediate host of the trematode Schistosoma mansoni. Our results highlight that duplication and domestication of an acquired bacterial toxin gene in the snail genome result in the acquisition of a novel and diversified toxin family.... |
| Tipo: Text |
Palavras-chave: Biomphalaria; Biomphalysin; Aerolysin; Invertebrate immunity; Pore-forming toxin (PFT); Structure. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00687/79909/82814.pdf |
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| Galinier, Richard; Roger, Emmanuel; Mone, Yves; Duval, David; Portet, Anais; Pinaud, Silvain; Chaparro, Cristian; Grunau, Christoph; Genthon, Clemence; Dubois, Emeric; Rognon, Anne; Arancibia, Nathalie; Dejean, Bernard; Theron, Andre; Gourbal, Benjamin; Mitta, Guillaume. |
| In recent decades, numerous studies have sought to better understand the mechanisms underlying the compatibility between Biomphalaria glabrata and Schistosoma mansoni. The developments of comparative transcriptomics, comparative genomics, interactomics and more targeted approaches have enabled researchers to identify a series of candidate genes. However, no molecular comparative work has yet been performed on multiple populations displaying different levels of compatibility. Here, we seek to fill this gap in the literature. We focused on B. glabrata FREPs and S. mansoni SmPoMucs, which were previously demonstrated to be involved in snail/schistosome compatibility. We studied the expression and polymorphisms of these factors in combinations of snail and... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00388/49951/50515.pdf |
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| Registros recuperados: 22 | |
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