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Innovative metabolomic workflows to study growth kinetics dinoflagellate cysts revived from modern and ancient sediments ArchiMer
Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf
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A macroscopic and stereological imaging dataset of Pleuronectes platessa ovaries ArchiMer
Sauger, Carine; Quinquis, Jerome; Kellner, Kristell; Heude-berthelin, Clothilde; Lepoittevin, Mélanie; Elie, Nicolas; Dubroca, Laurent.
The North Sea plaice, Pleuronectes platessa (Linnaeus, 1758), is a commonly studied commercial flatfish with poorly known ovarian histology. The following dataset is a collection of female plaice gonad images and their corresponding histological slides, collected during a complete season of the plaice’s reproduction cycle. Stereology was used to determine the percentage of different structures found throughout the ovaries. Inter-agent calibrations were accomplished in order to harmonize the stereological readings, and were based on a comprehensive reading protocol and histological lexicon that were specifically written for the plaice’s ovaries. The distribution and homogeneity of the different cell types found throughout the ovaries were also evaluated....
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00631/74342/73994.pdf
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An objective take on defining sexual maturity in the plaice, Pleuronectes platessa (Linné, 1758) ArchiMer
Sauger, Carine; Quinquis, Jerome; Lepoittevin, Mélanie; Heude-berthelin, Clothilde; Kellner, Kristell; Elie, Nicolas; Dubroca, Laurent.
The European plaice (Pleuronectes platessa) is a commercial species with stock management advice given through the estimation of fish length at maturity (L50) (Mahé et al., 2007). Estimating the maturity of fish species has been worked on by the International Council for the Exploration of the Sea (ICES) that wants to harmonize the definitions, terminologies and practices used to determine the different maturity phases. Project MATO (MATurité Objective des poissons par l’histologie quantitative) will, among other things, bring knowledge on the ovarian histology of the plaice in order to correlate histological maturity with macroscopic parameters like the size color and texture of the ovaries. With this knowledge, the aim of this study is to set up a...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00609/72093/70818.pdf
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Workflows novateurs pour une analyse métabolomique plus claire et plus intégrative ArchiMer
Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele.
During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "old" and 2006 for "modern". In total, 84 samples were obtained. Each experimental...
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00485/59662/62721.pdf
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Sélection de workflows adaptés à une analyse métabolomique plus transparente et intégrative ArchiMer
Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele.
During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "ancient" and 2006 for "modern". In total, 84 samples were obtained. Each experimental...
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00498/60941/64339.pdf
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Protocole de détermination des structures histologiques d’ovaires et de l’ovogenèse de la plie, Pleuronectes platessa (Linné, 1758) ArchiMer
Sauger, Carine; Quinquis, Jerome; Dubroca, Laurent; Kellner, Kristell; Lepoittevin, Mélanie; Heude Berthelin, Clothilde; Elie, Nicolas.
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00501/61235/64813.pdf
Registros recuperados: 6
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