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| Thonar, Cécile; Erb, A.; Jansa, J.. |
| Quantitative real-time PCR (qPCR) is slowly becoming established as a tool to quantify abundance of different arbuscular mycorrhizal fungal (AMF) taxa in roots and in soil. Here, we describe the development and field validation of qPCR markers (i.e. primers with associated hydrolysis probes), targeting taxon-specific motifs in the nuclear large ribosomal subunit RNA genes. Design of such markers is complicated by the multinuclear and multigenomic cellular organization of these fungi and the high DNA sequence diversity within the smallest biologically relevant units (i.e. single-spore isolates). These limitations are further compounded by inefficient biomass production of these fungi, resulting in limited availability of pure genomic DNA (gDNA) of... |
| Tipo: Journal paper |
Palavras-chave: Soil. |
| Ano: 2011 |
URL: http://orgprints.org/25988/1/Thonar_et_al_2011_CT33.pdf |
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| Thonar, C.; Erb, A.; Jansa, J.. |
| Quantitative real-time PCR (qPCR) is slowly becoming established as a tool to quantify abundance of different arbuscular mycorrhizal fungal (AMF) taxa in roots and in soil. Here, we describe the development and field validation of qPCR markers (i.e. primers with associated hydrolysis probes), targeting taxon-specific motifs in the nuclear large ribosomal subunit RNA genes. Design of such markers is complicated by the multinuclear and multigenomic cellular organization of these fungi and the high DNA sequence diversity within the smallest biologically relevant units (i.e. single-spore isolates). These limitations are further compounded by inefficient biomass production of these fungi, resulting in limited availability of pure genomic DNA (gDNA) of... |
| Tipo: Journal paper |
Palavras-chave: Indicators and other value-laden measures; Soil. |
| Ano: 2012 |
URL: http://orgprints.org/21817/1/thonar-etal-2012-MolecularEcol-Resources-Vol12-p219-232.pdf |
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