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	Provedor de dados Electron. J. Biotechnol. (2) Autor Espinoza,Juan Carlos (2) Campano,María Soledad (1) Eissler,Yoanna (1) Garland,David E (1) Kuznar,Juan (1) Pavlov,Maria Soledad (1) Robeson,James (1) Santander,Javier (1) Mais... Palavra-chave Digital image analysis (1) Fluorescence microscope methods (1) Immunofluorescence (1) Infectious pancreatic necrosis virus (IPNV) (1) SYBR-Green I (1) Tipo do documento Journal article (2) Ano 2011 (1) 2003 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Infection of Caenorhabditis elegans by Salmonella typhi Ty2 Electron. J. Biotechnol. Santander,Javier; Espinoza,Juan Carlos; Campano,María Soledad; Robeson,James. Several serovars of Salmonella infect and kill the nematode C. elegans. However, here we report that Salmonella typhi Ty2, a representative strain of this human pathogen, readily infects the intestinal lining of C. elegans without significantly affecting its viability. Our observation suggests extending the use of the C. elegans model system for the study of host parasite relationships, to address problems concerning the biology of S. typhi. Tipo: Journal article Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200008 Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects Electron. J. Biotechnol. Pavlov,Maria Soledad; Eissler,Yoanna; Espinoza,Juan Carlos; Garland,David E; Kuznar,Juan. A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody. Tipo: Journal article Palavras-chave: Digital image analysis; Fluorescence microscope methods; Immunofluorescence; Infectious pancreatic necrosis virus (IPNV); SYBR-Green I. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011 Registros recuperados: 2 Primeira ... 1 ... Última

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