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Isolation of Dickeya dadantii strains from potato disease and biocontrol by their bacteriophages BJM
Soleimani-Delfan,Abbas; Etemadifar,Zahra; Emtiazi,Giti; Bouzari,Majid.
One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dickeya dadantii; Soft rot; Bacteriophage; Antibiotic resistance; Biocontrol.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300791
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Isolation and characterization of novel thermophilic lipase-secreting bacteria BJM
Rabbani,Mohammed; Bagherinejad,Mohammad Reza; Sadeghi,Hamid MirMohammad; Shariat,Ziaedin Samsam; Etemadifar,Zahra; Moazen,Fatemeh; Rahbari,Manizheh; Mafakher,Ladan; Zaghian,Saeideh.
The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot spring, Persian Gulf, desert area and oil-contaminated soil, were analyzed for thermophilic extracellular-lipase producing organisms. Six strains with high activity on rhodamine B plates were selected for chemical identification and further study. Among these isolated bacteria, four strains show higher activity in pH-Stat method at 55 °C. These strains were identified by PCR amplification of 16s rRNA genes using universal primers. Fermentation increased the activity up to 50%. The growth medium, designed for lipase production, increased the activity up to 4.55 folds. The crude supernatant of ZR-5 after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular lipase; PH-stat method; Thermophilic lipase; Fermentation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400013
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