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Eggens, M; Galgani, Francois; Klungsoyr, J; Everts, J. |
During the Bremerhaven Workshop an improved, fast and easy method for the determination of ethoxyresorufin-O-deethylase (EROD) activity in dab Limanda limanda liver was tested. The improvement involved the use of a 12500 x g supernatant instead of the microsomal fraction, thus omitting the need for long ultra-centrifugations which cannot be carried out aboard a research vessel. EROD activities in the 12 500 x g supernatant and in the microsomal suspension were well correlated. Despite its disadvantages of lower specific EROD activity, higher minimum detection limit and higher analytical variance, the 12 500 x g supernatant was used for further analysis. EROD activity in dab liver showed a spatial trend corresponding to a pollution gradient in the German... |
Tipo: Text |
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Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00091/20247/17898.pdf |
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