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| Faria,Carla Bertechini; Almeida-Ferreira,Giovana Caputo; Gagliardi,Karina Bertechine; Alves,Tatiane Cristina Albuquerque; Tessmann,Dauri José; Machinski Junior,Miguel; Barbosa-Tessmann,Ione Parra. |
| The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bulgur wheat; Fusarium graminearum; PCR; Molecular detection; Food protection. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030 |
