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| Dias,R.P.; Pinheiro,R.R.; Andrioli,A.; Farias,A.C.; Sousa,A.L.M.; Azevedo,D.A.A.; Araújo,J.F.; Aguiar,T.D.F.; Teixeira,M.F.S.. |
| ABSTRACT This study aimed to isolate cells from the Wharton's jelly of umbilical cord (WJUC) of sheep collected during natural parturition using different culture media, in addition to reporting for the first time the permissiveness of these cells to in vitro infection by small ruminant lentiviruses. Ten umbilical cords were collected from healthy sheep. Each cord explants were grown in different media consisting of MEM, low glucose DMEM, M199, and RPMI-1640. The permissiveness of infection of sheep cells from WJUC was tested with CAEV-Cork and MVV-K1514 strains, inoculating 0.1 MOI of each viral strain. Four supernatants from each strain were obtained from WJUC sheep cell cultures infected in different media. The results demonstrated the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CAEV-Cork; MVV-K1514; Permissiveness; In vitro infection. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501292 |
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