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	Provedor de dados BJPS (1) Autor Ahmadzadeh,Maryam (1) Farshdari,Farzaneh (1) Mohit,Elham (1) Nematollahi,Leila (1) Palavra-chave Breast cancer (1) Expression (1) HER2 (1) ScFv (1) Solubility (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2020 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última The improvement of anti-HER2 scFv soluble expression in Escherichia coli BJPS Farshdari,Farzaneh; Ahmadzadeh,Maryam; Nematollahi,Leila; Mohit,Elham. The relationship between the expression of HER2 and malignity of breast tumors has led to the generation of antibodies targeting HER2+ tumors. In addition, the expression of scFvs, as the smallest antigen-binding region of antibody containing two disulfide bonds in Escherichia coli often results in accumulating non-functional protein in the cytoplasm. A redox-modified strain of E. coli such as Origami (DE3) may facilitate the formation of proper disulfide bond in cytoplasm. The present study aimed to optimize the expression of anti-HER2 scFv in Origami and evaluate the influence of induction temperature, and host strain on the solubility of the protein. To this aim, chemicallysynthesized anti-HER2 scFv of Trastuzumab was cloned in pET-22b (+). The results... Tipo: Info:eu-repo/semantics/article Palavras-chave: Breast cancer; HER2; ScFv; Expression; Solubility. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100519 Registros recuperados: 1 Primeira ... 1 ... Última

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