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Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome BJM
Martins,Maira de S.N.; Castro,Alessandra M.M.G. de; Lima,Michele dos S.; Pinto,Vivian da S.C.; Silva,Thaís G. da; Fava,Claudia Del; Depes,Claudio Regis; Okuda,Liria H.; Pituco,Edviges M..
Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovine herpesvirus type 2 (OvHV-2); Histopathology; Qualitative PCR; Quantitative PCR; Phylogenetic analysis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200366
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[RETRACTED ARTICLE] Bovine Vaccinia in dairy cattle and suspicion of vesicular disease on milkers in Brazil Ciência Rural
Silva,Thaís Garcia da; Lima,Michele dos Santos; Castro,Alessandra Marnie Martins Gomes de; Martins,Maira de Souza Nunes; Castiglioni,Vivian Cardoso; Fava,Claudia Del; Okuda,Liria Hiromi; Pituco,Edviges Maristela.
ABSTRACT: Bovine vaccinia (BV) is a vesicular disease induced by the Vaccinia virus (VACV) that affects milk production and is an occupational zoonosis. This research had the following objectives: (i) detection of VACV by qPCR in cattle with clinical suspicion of vesicular disease; (ii) symptoms characterization in animals and milkers with clinical suspicion of the disease and virus detection in humans; and (iii) identification of risk factors for infections of VACV in herds from several Brazilian states. A total of 471 bovine epithelial samples from dairy farms, in 15 Brazilian states, were evaluated between 2007 and 2012. The samples were tested by quantitative PCR (qPCR) using SYBR Green® reagents, validated with a lower limit of detection of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Orthopoxvirus; Poxviridae; Quantitative PCR; Risk factor; VACV; Zoonosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600455
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Bovine Vaccinia in dairy cattle and suspicion of vesicular disease on milkers in Brazil Ciência Rural
Silva,Thaís Garcia da; Lima,Michele dos Santos; Castro,Alessandra Marnie Martins Gomes de; Martins,Maira de Souza Nunes; Castiglioni,Vivian Cardoso; Fava,Claudia Del; Okuda,Liria Hiromi; Pituco,Edviges Maristela.
ABSTRACT: Bovine vaccinia (BV) is a vesicular disease induced by the Vaccinia virus (VACV) that affects milk production and is an occupational zoonosis. This research had the following objectives: (i) detection of VACV by qPCR in cattle with clinical suspicion of vesicular disease; (ii) symptoms characterization in animals and milkers with clinical suspicion of the disease and virus detection in humans; and (iii) identification of risk factors for infections of VACV in herds from several Brazilian states. A total of 471 bovine epithelial samples from dairy farms, in 15 Brazilian states, were evaluated between 2007 and 2012. The samples were tested by quantitative PCR (qPCR) using SYBR Green® reagents, validated with a lower limit of detection of 100...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Orthopoxvirus; Poxviridae; Quantitative PCR; Risk factor; VACV; Zoonosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500451
Registros recuperados: 3
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