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	Provedor de dados BJM (2) Autor Ashayeri-Panah,Mitra (2) Feizabadi,Mohammad Mehdi (2) Eftekhar,Fereshteh (1) Eftekhar,Freshteh (1) Ghamsari,Maryam Mobarak (1) Mobarak-Qamsari,Maryam (1) Parvin,Mahmood (1) Mais... Palavra-chave Klebsiella pneumoniae (2) Extended-spectrum β-lactamase (1) Genotyping (1) Integron (1) Multiple antibiotic resistance (1) Pulsed field gel electrophoresis (1) Random amplified polymorphic DNA (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA BJM Ashayeri-Panah,Mitra; Eftekhar,Fereshteh; Ghamsari,Maryam Mobarak; Parvin,Mahmood; Feizabadi,Mohammad Mehdi. In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed sufficient discriminatory power (DI > 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with PFGE. Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Pulsed field gel electrophoresis; Random amplified polymorphic DNA; Genotyping. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300024 Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae BJM Mobarak-Qamsari,Maryam; Ashayeri-Panah,Mitra; Eftekhar,Freshteh; Feizabadi,Mohammad Mehdi. The present study describes integron mediated multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of Klebsiella pneumoniae. One hundred and four clinical isolates of K. pneumoniae from two Iranian hospitals were screened for extended-spectrum β-lactamase production and susceptibility of the extended-spectrum β-lactamase producing isolates was determined to 17 antibiotics by disc diffusion. Presence of integron classes 1, 2 and 3 was detected by PCR and integrase specific primers. Isolates harboring class 1 integron were then screened for variable regions using PCR. Fifty isolates (48%) produced extended-spectrum β-lactamases among which, 22 (44%) harbored class 1, 3 (6%) carried class 2 and none contained class 3... Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Extended-spectrum β-lactamase; Multiple antibiotic resistance; Integron. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300028 Registros recuperados: 2 Primeira ... 1 ... Última

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