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| Fereig, Ragab M.; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; AbouLaila, Mahmoud Rezk; Guswanto, Azirwan; Thu-Thuy Nguyen; Mohamed, Adel Elsayed Ahmed; Inoue, Noboru; Igarashi, Ikuo; Nishikawa, Yoshifumi. |
| Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginate infections cause serious diseases in cattle, and are primarily transmitted by arthropod vectors (ticks for B. bovis, B. bigemina, and A. marginate and various types of flies for T. evansi). In the last few years, there have been many reports of a high prevalence of certain protozoan infections in northern Egypt, but no accurate or adequate data are available for the southern regions. Therefore, in this study, we screened for evidence of such diseases in economically important cattle species using serum samples. The seroprevalence of protozoan infections in cattle was determined with enzyme-linked immunosorbent assays using species-specific diagnostic antigens. In a total of 301 cattle... |
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Palavras-chave: Babesia bovis; Babesia bigemina; Trypanosoma evansi; Anaplasma marginale; Cattle; Egypt. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4579 |
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| Fereig, Ragab M.; Nishikawa, Yoshifumi. |
| Toxoplasmosis remains a life-threatening infection of humans and various domestic and wild animals worldwide. It is caused by the obligatory intracellular protozoan parasite Toxoplasma gondii. Peroxiredoxins (Prxs) are a family of antioxidant enzymes that protect cells from oxidative stress from hydroperoxides. In the recent years, several studies have reported the potential use of T. gondii-derived enzymes in triggering protective immunity against T. gondii infection. Therefore, this study was conducted to investigate the immunogenicity and protective efficacy of TgPrx3. In vitro stimulation of peritoneal macrophages with recombinant TgPrx3 protein fused to glutathione-S transferase (TgPrx3-GST) enhanced IL-12p40 production, indicating the... |
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Palavras-chave: Toxoplasma gondii; Peroxiredoxin 3; Vaccine; Immunization. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4569 |
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| Fereig, Ragab M.; AbouLaila, Mahmoud Rezk; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; Ali, Alsagher O.; Ali, Asmaa F.; Hilali, Mosaad; Zaid, Anis; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi. |
| Neospora caninum and Cryptosporidium parvum are intracellular protozoan parasites that are distributed worldwide and of major economical concern in cattle industry. N. caninum can cause abortion storms and high culling rates, whereas C. parvum has zoonotic implications and can cause diarrhea in calves. There are currently no data on the prevalence of neosporosis and cryptosporidiosis in humans or animals in southern Egypt. Prevalence of these two infections was determined in a sample of cattle from two different areas in southern Egypt, Sohag and Qena, using enzyme-linked immunosorbent assay. A total 301 cattle were sampled, of which 18.9% were positive for N. caninum, 35.9% were positive for C parvum and 10.0% were positive for both. Geographical location... |
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Palavras-chave: Cattle; Cryptosporidium parvum; Neospora caninum; ELISA; Egypt. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4531 |
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| Fereig, Ragab M.; Mahmoud, Hassan Y.A.H.; Mohamed, Samy G.A.; AbouLaila, Mahmoud Rezk; Abdel-Wahab, Azza; Osman, Salama Ahmed; Zidan, Sherif Abdallah; El-Khodary, Sabry A.; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi. |
| Toxoplasmosis is a cosmopolitan protozoan disease that has been recorded in a wide range of vertebrate hosts, including humans. In response to the paucity of available data, this study was undertaken to comprehensively establish the seroprevalence of Toxoplasma gondii among various farm animals in different localities of Egypt. The latex agglutination test and TgGRA7-based enzyme-linked immunosorbent assay were used to screen the investigated animals for anti-T. gondii IgG antibodies. When only samples with simultaneously positive results for both the latex agglutination test and the TgGRA7-based ELISA were considered positive, 174 (26.7%) of 652 serum samples from different animals were seropositive. The prevalence of antibodies according to species was:... |
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Palavras-chave: Egypt; Seroprevalence; Toxoplasma gondii; Toxoplasmosis. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4545 |
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| Fereig, Ragab M.; Kuroda, Yasuhiro; Terkawi, Mohamad Alaa; Mahmoud, Motamed Elsayed; Nishikawa, Yoshifumi. |
| To develop a vaccine against Toxoplasma gondii, a vaccine antigen with immune-stimulating activity is required. In the present study, we investigated the immunogenicity and prophylactic potential of T. gondii peroxiredoxin 1 (TgPrx1). The TgPrx1 was detected in the ascitic fluid of mice 6 days postinfection, while specific antibody levels were low in the sera of chronically infected mice. Treatment of murine peritoneal macrophages with recombinant TgPrx1 triggered IL-12p40 and IL-6 production, but not IL-10 production. In response to TgPrx1, activation of NF-kappa B and IL-6 production were confirmed in mouse macrophage cell line (RAW 264.7). These results suggest the immune-stimulating potentials of TgPrx1. Immunization of mice with recombinant TgPrx1... |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4462 |
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