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| Andrade,Antero Silva Ribeiro de; Fernandes,Octávio; Heub,Marcia; Carvalho,Maria de Lourdes Ribeiro; Fontes,Cor Jesus; Melo,Maria Norma de. |
| DNA hybridisation, using probes labelled with 32P, was used to type Leishmania samples isolated from patients living in endemic areas of Mato Grosso State (Brazil), and clinically diagnosed as having tegumentary leishmaniasis. kDNA cloned mini-circle probes specific for the Leishmania mexicana and Leishmania braziliensis complexes were used. The results showed that L. braziliensis is the predominant group infecting human patients in the state. Sixty-eight samples were typed, 64 samples (94.1%) belonging to the L. braziliensis complex and only four (5.9%) belonging to the L. mexicana complex. Accurate identification of the Leishmania permits better orientation of the medical follow-up, since clinical manifestations may vary depending on the complex to which... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Leishmaniasis; DNA hybridisation; Mato Grosso state. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700030 |
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| Volotão,Aline Cardoso Caseca; Ramos,Nathália Motta Delvaux; Fantinatti,Maria; Moraes,Marcus Vinícius Proença de; Atique Netto,Halim; Storti-Melo,Luciane Moreno; Godoy,Elenir Alves Macedo de; Rossit,Andréa Regina Baptista; Fernandes,Octávio; Machado,Ricardo Luiz Dantas. |
| OBJECTIVE: In order to evaluate the potential zoonotic transmission of Giardia duodenalis, isolates from humans and dogs in the Northwestern region of the São Paulo State, Brazil were characterized based on the β-giardin gene. METHODS: The samples were analyzed by sequencing of the Nested-PCR products. RESULTS: The A1 and A2 subgenotypes were detected in human and dogs. Cysts of assemblage B, C and D have not been found in any isolates studied. CONCLUSIONS: These results are consistent with the view that giardiasis in the largest endemic region of the Brazil should not be seen as a single entity. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Giardia duodenalis; Beta giardin gene; Genotypes; Zoonosis; Brazil. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400014 |
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