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| Gisbert, Enric; Skalli, Ali; Fernandez, Ignacio; Kotzamanis, Yannis; Zambonino, Jose Luiz; Fabregat, Rogelio. |
| In this study, we have evaluated the incorporation of two types of protein hydrolysates at 9 and 12% levels of inclusion, one from yeast (Saccharomyces cerevisiae, YPH) and another one from pig blood (PBPH), in microdiets for gilthead sea bream (Sparus aurora) larvae, and compared these results to a microdiet containing fish protein hydrolysate and another group only fed with enriched live prey (rotifers and Artemia). The trial consisted in substituting up to 75% (wt/wt) the enriched Artemia with the experimental microdiets from 15 to 40 days post-hatch, whereas larvae were exclusively fed on microdiets from 40 to 55 dph. Protein hydrolysates used in the present study were obtained from different raw materials (yeast, pig blood and fish protein... |
| Tipo: Text |
Palavras-chave: Digestive enzymes; Larvae; Gilthead sea bream; Protein hydrolysate; Skeletal deformities; Sparus aurata. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00085/19584/17467.pdf |
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| Fernandez, Ignacio; Pimentel, Marta S.; Ortiz-delgado, Juan B.; Hontoria, Francisco; Sarasquete, Carmen; Estevez, Alicia; Zambonino, Jose-luis; Gisbert, Enric. |
| The effects of different levels of vitamin A (VA) in Senegalese sole larval performance and development were evaluated by means of a dietary dose-response experiment using enriched Artemia metanauplii as a carrier of this micronutrient. Larvae were fed from 6 to 27 days post hatch (dph) with enriched Artemia containing graded levels of total VA (1.3, 2.1, 4.5 and 12.9 mu g VA mg(-1) DW). The content of VA in live prey directly affected its accumulation in larvae and early juveniles. Retinyl palmitate accumulated during larval ontogeny, whereas retinol showed the opposite trend, decreasing from hatching until 41 dph and then remaining constant until the end of the study. Inmetamorphic larvae (10 and 15 dph), VA did not affect the number of thyroid follicles... |
| Tipo: Text |
Palavras-chave: Deformities; Thyroid hormones; Skeleton; Vitamin A; Larval quality; Solea senegalensis; Senegalese sole. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7385.pdf |
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| Fernandez, Ignacio; Darias, Maria; Andree, Karl B.; Mazurais, David; Zambonino, Jose-luis; Gisbert, Enric. |
| Background: Vitamin A (VA) has a key role in vertebrate morphogenesis, determining body patterning and growth through the control of cell proliferation and differentiation processes. VA regulates primary molecular pathways of those processes by the binding of its active metabolite (retinoic acid) to two types of specific nuclear receptors: retinoic acid receptors (RARs) and retinoid X receptors (RXRs), which promote transcription of downstream target genes. This process is well known in most of higher vertebrates; however, scarce information is available regarding fishes. Therefore, in order to gain further knowledge of fish larval development and its disruption by nutritional VA imbalance, the relative expression of some RARs and RXRs, as well as several... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14264/11546.pdf |
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| Fernandez, Ignacio; Lopez-joven, Maria-carmen; Andree, Karl B.; Roque, Ana; Gisbert, Enric. |
| Senegalese sole (Solea senegalensis) has been considered since the 1990´s to be a promising flatfish species for diversifying European marine aquaculture. However, pathogen outbreaks leading to high mortality rates can impair Senegalese sole commercial production at the weaning phase. Different approaches have been shown to improve fish immunocompetence; with this in mind the objective of the work described herein was to determine whether increased levels of dietary vitamin A (VA) improve the immune response in early juveniles of Senegalese sole. For this purpose, Senegalese sole were reared and fed with Artemia metanauplii containing increased levels of VA (37,000; 44,666; 82,666 and 203,000 total VA IU Kg-1) from 6 to 60 days post-hatch (early juvenile... |
| Tipo: Text |
Palavras-chave: Vitamin; Retinoic acid; Immune system; Gene expression; Senegalese sole Solea senegalensis; Photobacterium damselae subp. damselae. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38617/37147.pdf |
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