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	Provedor de dados BABT (2) Ciência Rural (1) Genet. Mol. Biol. (1) Autor Fernandez,Maria Aparecida (4) Silva,José Luis da Conceição (2) Bravo,Juliana Pereira (1) Falco,José Ricardo Penteado (1) Felipes,Joice (1) Gandra,Eliezer Avila (1) Mikami,Liya Regina (1) Polinarski,Celso Aparecido (1) Saccuti,Cecília Ferreira (1) Silva,Jorge Adolfo (1) Silva,Wladimir Padilha da (1) Soares,Maria Albertina de Miranda (1) Zanatta,Daniela Bertolini (1) Mais... Palavra-chave Bradysia hygida (2) Brazilian flies (1) DNA loops (1) Diatraea saccharalis (1) Feulgen reaction (1) Genome size (1) Insect polytene cells (1) Lepidoptera (1) MtDNA Control Region (1) Multiplex PCR (1) Nuc gene (1) Nuclear halo (1) Nuclear matrix (1) Rhynchosciara americana (1) Salivary gland (1) Sciaridae (1) Staphylococcus aureus (1) Staphylococcus hyicus (1) Staphylococcus intermedius (1) Sugarcane borer (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/other (1) Ano 2016 (1) 2008 (1) 2005 (2) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Genome size of three Brazilian flies from the Sciaridae family Genet. Mol. Biol. Saccuti,Cecília Ferreira; Soares,Maria Albertina de Miranda; Falco,José Ricardo Penteado; Fernandez,Maria Aparecida. We determined the genome size of the Brazilian sciarid flies Bradysia hygida, Rhynchosciara americana and Trichosia pubescens (Diptera: Sciaridae) using absorbance measurements of Feulgen-stained nuclei belonging to these species (and chicken erythrocytes as a standard) to calculate the amount of DNA in picograms (pg) and the number of base pairs (bp), or C-value, for each of these species. The C-values were: 3 x 10(8) bp (0.31 pg) for B. hygida; 3.6 x 10(8) bp (0.37 pg) for R. americana; and 1 x 10(9) bp (1.03 pg) for T. pubescens. The sciarids investigated in this work had considerably higher C-values than the average for previously described dipteran species, including D. melanogaster. Tipo: Info:eu-repo/semantics/other Palavras-chave: Genome size; Feulgen reaction; Sciaridae; Bradysia hygida; Rhynchosciara americana; Trichosia pubescens; Brazilian flies. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500015 Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells BABT Polinarski,Celso Aparecido; Silva,José Luis da Conceição; Mikami,Liya Regina; Fernandez,Maria Aparecida. A protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles. Tipo: Info:eu-repo/semantics/article Palavras-chave: Insect polytene cells; Nuclear matrix; Nuclear halo; DNA loops; Salivary gland; Bradysia hygida. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300004 Sequence and analysis of the mitochondrial DNA control region in the sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae) BABT Bravo,Juliana Pereira; Felipes,Joice; Zanatta,Daniela Bertolini; Silva,José Luis da Conceição; Fernandez,Maria Aparecida. This study aimed at the sequence and analysis of the mtDNA control region (CR) of the Diatraea saccharalis. The genome PCR amplification was performed using the complementary primers to the flanking regions of Bombyx mori CR mitochondrial segment. The sequencing revealed that the amplified product was 568 bp long, which was smaller than that observed for B. mori (725 bp). Within the amplified segment, a sequence with 338 nucleotides was identified as the control region, which displayed a high AT content (93.5%). The D. saccharalis mtDNA CR multiple sequence alignment analysis showed that this region had high similarity with the Lepidoptera Cydia pomonella. Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA Control Region; Diatraea saccharalis; Lepidoptera; Sugarcane borer. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400004 Detection by multiplex PCR of Staphylococcus aureus , S. intermedius and S. hyicus in artificially contaminated milk Ciência Rural Gandra,Eliezer Avila; Fernandez,Maria Aparecida; Silva,Jorge Adolfo; Silva,Wladimir Padilha da. ABSTRACT: This research aimed to detect coagulase-positive Staphylococcus (CPS) directly in samples of artificially contaminated milk, using multiplex PCR (mPCR). Standard and isolated bacterial strains of S. aureus, S. epidermidis, S. hyicus, S. intermedius, Listeria monocytogenes and Escherichia coli species were used, evaluating the specificity and detection limit of mPCR, for artificially contaminated UHT milk. Primers specific for the nuc gene (NUC1-NUC2 were used for S. aureus, NUC3-NUC4 for S. hyicus and NUC5-NUC6 for S. intermedius). It was possible to detect the three target species by mPCR, directly from bovine whole milk, with adequate specificity and acceptable detention limit for identification of coagulase-positive Staphylococcus (CPS) in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Staphylococcus hyicus; Staphylococcus intermedius; Multiplex PCR; Nuc gene. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801418 Registros recuperados: 4 Primeira ... 1 ... Última

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