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| Nora,Luísa Czamanski; Gonçales,Relber Aguiar; Martins-Santana,Leonardo; Ferreira,Beatriz Henriques; Rodrigues,Fernando; Silva-Rocha,Rafael. |
| Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation:... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Synthetic biology; Fungi; Agrobacterium tumefaciens; Transformation; Vectors.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300395 |
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