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	Provedor de dados BABT (1) Autor Evangelista,Alex Ferreira (1) Ferreira,Graziela Batista (1) Jordão,Elizabete (1) Santana,José Carlos Curvelo (1) Severo Junior,João Baptista (1) Souza,Roberto Rodrigues de (1) Tambourgi,Elias Basile (1) Mais... Palavra-chave Aqueous two-phase systems (1) CaCl2 (1) Maize malt (1) Optimization (1) PEG 6000 (1) Partitioning (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2007 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2 BABT Ferreira,Graziela Batista; Evangelista,Alex Ferreira; Severo Junior,João Baptista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile; Jordão,Elizabete. This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was about 4.2 and was achieved in ATPS in a single purification step. The theoretical maximum partition coefficient was between 4.1-4.3. The process was very suitable... Tipo: Info:eu-repo/semantics/article Palavras-chave: Partitioning; Optimization; Aqueous two-phase systems; Maize malt; PEG 6000; CaCl2. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300020 Registros recuperados: 1 Primeira ... 1 ... Última

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