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| Degrandi,Tiago Marafiga; Pita,Sebastian; Panzera,Yanina; Oliveira,Edivaldo Herculano C. de; Marques,José Ribamar Felipe; Figueiró,Marivaldo Rodrigues; Marques,Larissa Coêlho; Vinadé,Lucia; Gunski,Ricardo José; Garnero,Analía Del Valle. |
| Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 18S rDNA; Bubalus bubalis; FISH. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300009 |
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