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	Provedor de dados J. Venom. Anim. Toxins incl. Trop. Dis. (1) Autor Bressan,W. (1) Figueiredo,J. E. F. (1) Gomez,M. V. (1) Kalapothakis,E. (1) Palavra-chave Bioinsecticide (1) Escherichia coli (1) Heterologous expression (1) Molecular cloning (1) Spodoptera frugiperda (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2008 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Heterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer) J. Venom. Anim. Toxins incl. Trop. Dis. Figueiredo,J. E. F.; Kalapothakis,E.; Gomez,M. V.; Bressan,W.. Insect-pests are global problems that cause severe damage to crop plants, and their control is commonly based on chemical insecticides. However, negative effects of pesticides on the environment and human health emphasize the necessity to develop alternative methods for insect-pest control. In the present study, a gene coding for the insecticidal peptide TX4(6-1) of the Brazilian armed spider (Phoneutria nigriventer) was cloned in fusion with maltose binding protein (MBP) and expressed in Escherichia coli. The affinity purified protein MBP-GlyTX4 was cleaved with the Xa factor and used for a bioassay against Spodoptera frugiperda and rabbit immunization. Five micrograms GlyTX4 protein injected into the hemocoel of larvae and abdominal cavity of adults... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioinsecticide; Molecular cloning; Heterologous expression; Escherichia coli; Spodoptera frugiperda. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200006 Registros recuperados: 1 Primeira ... 1 ... Última

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