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| Pasqual,Moacir; Dutra,Leonardo Ferreira; Araujo,Aparecida Gomes de; Figueiredo,Milene Alves de. |
| The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo culture; Nutrient medium; Breeding. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 |
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| Figueiredo,Milene Alves de; Pasqual,Moacir; Araujo,Aparecida Gomes de; Junqueira,Keize Pereira; Santos,Flávia Carvalho; Rodrigues,Vantuil Antônio. |
| Plantas de Cattleya loddigesii com 1,0-1,5cm de comprimento, oriundas de sementes germinadas in vitro, foram inoculadas nos tratamentos, os quais consistiram da adição de diferentes concentrações de cloreto e sulfato de potássio (ambos a 0, 125, 250, 375 e 500mg L-1) ao meio Knudson C, em todas as combinações possíveis, acrescido de 2g L-1 de carvão ativado e 150g L-1 de polpa de banana "Nanica". O meio teve seu pH ajustado para 5,8±0,1 e foi solidificado com 5g L-1 de ágar antes da autoclavagem a 121°C por 20 minutos. Após a inoculação, as culturas foram mantidas por 90 dias em sala de crescimento com irradiância em torno de 35µmol m-2 s-1, temperatura de 25±1°C e fotoperíodo de 16 horas. A combinação de 500mg L-1 de KCl com 500mg L-1 de K2SO4 promoveu... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orchidaceae; Cultura de tecidos; Cloreto de potássio; Sulfato de potássio. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000100043 |
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| Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene Alves de. |
| This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo culture; Tissue culture; Biotechnology; Citrus breeding. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031 |
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