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| Rossi,Daise Aparecida; Fonseca,Belchiolina Beatriz; Melo,Roberta Torres de; Felipe,Gutembergue da Silva; Silva,Paulo Lourenço da; Mendonça,Eliane Pereira; Filgueiras,Ana Luzia Lauria; Beletti,Marcelo Emilio. |
| Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter coli; Viability; Transmission; Breeder hens. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200014 |
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| Caruso,Lucilla Imbroinise Azeredo; Souza,Mauro Carlos Lopes; Filgueiras,Ana Luzia Lauria; Duque,Sheila Silva; Esteves,Wagner Thadeu; Thomé,Jaqueline Dark. |
| Campylobacteriosis is an infection frequently acquired through the consumption of animal origin products. Chicken can be considered the main responsible cause in the transmission chain of this disease. Ionizing radiation was used to verify the reduction of the microbiological load of Campylobacter jejuni present in chicken liver, which, in natura, can present contamination in up to 100% of the cases. The doses of irradiation used were: 0.20 kGy, 0.27 kGy, 0.30 kGy and 0.35 kGy. The samples of chicken liver were acquired in aviaries, local supermarkets and large chain supermarkets. The samples were analyzed for Campylobacter at FIOCRUZ. Irradiation was performed at COPPE/UFRJ, using a Gamma Cell Irradiator with a 60Co gamma source. Only the frozen sample... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food infection; Ionizing radiation; Campilobacteriosis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000200016 |
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