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	Provedor de dados Arq. Bras. Med. Vet. Zootec. (2) BJMBR (1) Autor Flatschart,R.B. (3) Abreu,J.T. (2) Martins,N.R.S. (2) Oliveira,D.D. (2) Resende,J.S. (2) Flatschart,A.V.F. (1) Folgueras-Flatschart,A.V. (1) Sogayar,M.C. (1) Mais... Palavra-chave Antisense (1) Codorna japonesa (1) Doença de Newcastle (1) ELISA (1) Expression interference (1) Gene function (1) Genetic knock-out (1) IgG (1) Ribozymes (1) Transgene overexpression (1) Transgenic cells and animals (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/other (1) Ano 2010 (1) 2007 (1) 1999 (1) País Brazil (3) Idioma Português (2) Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Purificação de IgG de codorna a partir de soro e gema de ovos Arq. Bras. Med. Vet. Zootec. Oliveira,D.D.; Resende,J.S.; Flatschart,A.V.F.; Abreu,J.T.; Flatschart,R.B.; Martins,N.R.S.. Tipo: Info:eu-repo/semantics/article Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200034 ELISA indireto para detecção de IgG antivírus da doença de Newcastle em soro de codorna Arq. Bras. Med. Vet. Zootec. Oliveira,D.D.; Folgueras-Flatschart,A.V.; Flatschart,R.B.; Resende,J.S.; Abreu,J.T.; Martins,N.R.S.. An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100%). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific... Tipo: Info:eu-repo/semantics/other Palavras-chave: Codorna japonesa; ELISA; IgG; Doença de Newcastle. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500040 Functional analysis of newly discovered growth control genes: experimental approaches BJMBR Flatschart,R.B.; Sogayar,M.C.. A large number of DNA sequences corresponding to human and animal transcripts have been filed in data banks, as cDNAs or ESTs (expression sequence tags). However, the actual function of their corresponding gene products is still largely unknown. Several of these genes may play a role in regulation of important biological processes such as cell division, differentiation, malignant transformation and oncogenesis. Elucidation of gene function is based on 2 main approaches, namely, overexpression and expression interference, which respectively mimick or suppress a given phenotype. The currently available tools and experimental approaches to gene functional analysis and the most recent advances in mass cDNA screening by functional analysis are discussed. Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene function; Transgene overexpression; Expression interference; Antisense; Ribozymes; Transgenic cells and animals; Genetic knock-out. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700011 Registros recuperados: 3 Primeira ... 1 ... Última

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