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| Mulero, Stephen; Boissier, Jérôme; Allienne, Jean‐françois; Quilichini, Yann; Foata, Joséphine; Pointier, Jean‐pierre; Rey, Olivier. |
| Under ongoing climate changes, the development of large‐scale monitoring tools for assessing the risk of disease emergence constitutes an urging challenge. This is particularly the case for snail‐borne diseases such as the urogenital bilharziasis that emerged in Corsica and threat European countries. The expansion of this tropical disease mainly relies on the local presence of competent snail hosts such as Bulinus truncatus. Unfortunately, very little is known about the actual repartition of freshwater snails worldwide which makes new emergences difficult to predict. In this study, we developed two ready‐to‐use environmental DNA‐based methods for assessing the distribution of B. truncatus from water samples collected in the field. We used two approaches, a... |
| Tipo: Text |
Palavras-chave: Bulinus truncatus; Corsica; DdPCR; Environmental DNA; Environmental monitoring; QPCR; Schistosomiasis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00593/70554/68723.pdf |
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| Mulero, Stephen; Toulza, Eve; Loisier, Anaïs; Zimmerman, Meryl; Allienne, Jean-françois; Foata, Joséphine; Quilichini, Yann; Pointier, Jean-pierre; Rey, Olivier; Boissier, Jérôme. |
| To assess the effect of anthropogenic activities on ecosystems, it is of prime importance to develop new tools enabling a rapid characterization of ecological communities. Freshwater ecosystems are particularly impacted and threatened by human activities and need thorough attention to preserve their biodiversity and the ecological services they provide. Studying such ecosystems is generally difficult because the associated organisms are hard to sample and to monitor. We present a ready-to-use environmental metabarcoding protocol to characterize and monitor the freshwater gastropods communities from water samples. The efficiency of this new tool was compared to a classical malacological survey at 19 sampled sites from 10 distinct rivers distributed over... |
| Tipo: Text |
Palavras-chave: Biomonitoring; Corsica; Environmental DNA; Malacology; Metabarcoding; Snail communities. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00666/77777/79917.docx |
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