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	Provedor de dados BJID (1) BJM (1) Autor Fonseca,André Salvador Kazantzi (2) Ikuta,Nilo (2) Lunge,Vagner Ricardo (2) Boeira,Thaís da Rocha (1) Celmer,Álvaro José (1) Coser,Janaina (1) Fraga,Aline Padilha (1) Marques,Edmundo Kanan (1) Vargas,Tatiana de (1) Mais... Palavra-chave DNA probes (1) HPV polymerase chain reaction polymorphism (1) Mycoplasma gallisepticum (1) Mycoplasma synoviae (1) Real-time PCR (1) Restriction fragment length (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Human papillomavirus detection and typing using a nested-PCR-RFLP assay BJID Coser,Janaina; Boeira,Thaís da Rocha; Fonseca,André Salvador Kazantzi; Ikuta,Nilo; Lunge,Vagner Ricardo. BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS: Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay. RESULTS: The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV,... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA probes; HPV polymerase chain reaction polymorphism; Restriction fragment length. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500009 A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks BJM Fraga,Aline Padilha; Vargas,Tatiana de; Ikuta,Nilo; Fonseca,André Salvador Kazantzi; Celmer,Álvaro José; Marques,Edmundo Kanan; Lunge,Vagner Ricardo. Mycoplasma gallisepticum (MS) and Mycoplasma synoviae (MS) are important avian pathogens and cause economic losses to the poultry industry. Molecular biology techniques are currently used for a rapid detection of these pathogens and the adoption of control measures of the diseases. The aim of this study was to develop and validate a technique for simultaneous detection of MG and MS by multiplex real time polymerase chain reaction (PCR). The complete assay (Multiplex MGMS) was designed with primers and probes specific for each pathogen and developed to be carried out in a single tube reaction. Vaccines, MG and MS isolates and DNA from other Mycoplasma species were used for the development and validation of the method. Further, 78 pooled clinical samples... Tipo: Info:eu-repo/semantics/article Palavras-chave: Real-time PCR; Mycoplasma gallisepticum; Mycoplasma synoviae. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200028 Registros recuperados: 2 Primeira ... 1 ... Última

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