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Silva,Cinthia Aparecida de Andrade; Lacerda,Maria Priscila Franco; Leite,Rodrigo Simões Ribeiro; Fonseca,Gustavo Graciano. |
Background: Enzyme production by solid state bioprocess (SSB) using residues as substrate for microorganisms is an alternative for costs reduction and to avoid their disposal into environment. The aim of this work was to evaluate the physiology of the fungus Lichtheimia ramosa in terms of microbial growth and production of amylases, β-glucosidases, carboxymethylcellulase (CMCase), and xylanases, via SSB, utilizing wastes of the Brazilian savannah fruits bocaiuva (Acrocomia aculeata), guavira (Campomanesia pubescens) and pequi (Caryocar brasiliense) as substrate at different temperatures (25, 30, and 35ºC) during 168 hrs. Results: Samples were taken every 24 hrs, which resulted in 8-points kinetic experiments to determine microbiological and... |
Tipo: Journal article |
Palavras-chave: Amylase β -glucosidase CMCase Lichtheimia ramosa xylanase. |
Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500009 |
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Garcia,Nayara Fernanda Lisboa; Santos,Flávia Regina da Silva; Gonçalves,Fabiano Avelino; Paz,Marcelo Fossa da; Fonseca,Gustavo Graciano; Leite,Rodrigo Simões Ribeiro. |
Background β-Glucosidases catalyze the hydrolysis of cellobiose and cellodextrins, releasing glucose as the main product. This enzyme is used in the food, pharmaceutical, and biofuel industries. The aim of this work is to improve the β-glucosidase production by the fungus Lichtheimia ramosa by solid-state fermentation (SSF) using various agroindustrial residues and to evaluate the catalytic properties of this enzyme. Results A high production of β-glucosidase, about 274 U/g of dry substrate (or 27.4 U/mL), was obtained by cultivating the fungus on wheat bran with 65% of initial substrate moisture, at 96 h of incubation at 35°C. The enzymatic extract also exhibited carboxymethylcellulase (CMCase), xylanase, and... |
Tipo: Journal article |
Palavras-chave: Cellobiase; Cellulases and hemicellulases; Industrial enzymes; Microbial enzymes. |
Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400010 |
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