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Fonseca,Isabela; Silva,Priscila Vendramini; Lange,Carla Christine; Guimarães,Marta F.M.; Weller,Mayara Morena Del Cambre Amaral; Sousa,Katiene Régia Silva; Lopes,Paulo Sávio; Guimarães,José Domingos; Guimarães,Simone E.F.. |
In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytokine; Gene expression; Immune response; Real-time PCR. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400018 |
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Peixoto,Jane de Oliveira; Faria,Danielle Assis de; Silva,Priscila Vendramini; Fonseca,Isabela; Lopes,Paulo Sávio; Guimarães,Simone Eliza Facioni. |
It was investigated the association between leptin gene single nucleotide polymorphisms (SNPs) T2411C and T3266G with carcass traits in F2 pigs obtained by crossing native Brazilian Piau with commercial sows composed of Landrace, Large White and Pietrain breeds. Association analyses were performed using a statistical model that included genotype, sex, and batch as fixed effects, sire and error as random effects. The T2411C SNP was associated with skinless and fatless boston shoulder weight (BSW), backfat thickness at last rib, 6.5 cm from the midline (P2), skinless and fatless picnic shoulder weight, and sirloin weight (SLW). The T3266G mutation was associated with slaughter age, bacon weight, BSW, midline backfat thickness between last and last but one... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidate gene; Pig genomics; Pig production. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000200008 |
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